Development of a Micro HG-PRT Activity Assay: Preliminary Complementation Studies with Lesch — Nyhan Cell Strains

  • P. Hösli
  • C. H. M. M. de Bruyn
  • T. L. Oei
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41)


The main purpose of the present study was to look with the heteropolykaryontest (ref. 1,2) for genetic complementation between different HG-PRT deficient cell strains, which had been fused and cultured in Plastic Film Dishes (PFD’s ref, 3,4) A radiochemical HG-PRT microassay, based on the use of the Parafilm Micro Cuvette (PMC; ref. 3,4) was developed, which permits the quantification of the enzyme activity at the single cell level.


Cell Strain Plastic Film Single Cell Level Genetic Complementation Normal Amniotic Fluid 
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  1. 1.
    P. Hösli: (1972) Microtechniques for the detection of intergenic and interallelic complementation in man. Bull. Europ. Soc. Hum. Genet, p.32.Google Scholar
  2. 2.
    P. Hösli: (1973) Inborn lysosomal disease: detection of genetic heterogeneity by complementation studies. Proceedings of the “Third International Research Conference on Lysosomes in Cell Pathology”, Louvain, in press.Google Scholar
  3. 3.
    P. Hösli: (1972) Tissue cultivation on plastic films. Tecnomara A.G., Rieterstrasse 59, Postfach CH 8059, Zurich, Zwitserland.Google Scholar
  4. 4.
    P. Hösli: (1972) Micro techniques for rapid prenatal diagnosis of inborn errors of metabolism in early pregnancy. Prenatal diagnosis newsletter 1 (1972) 10.Google Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • P. Hösli
    • 1
  • C. H. M. M. de Bruyn
    • 2
  • T. L. Oei
    • 2
  1. 1.Department of Human GeneticsAmsterdamThe Netherlands
  2. 2.Department of Human Genetics, Faculty of MedicineUniversity of NijmegenNijmegenThe Netherlands

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