Development of a Micro HG-PRT Activity Assay: Preliminary Complementation Studies with Lesch — Nyhan Cell Strains
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The main purpose of the present study was to look with the heteropolykaryontest (ref. 1,2) for genetic complementation between different HG-PRT deficient cell strains, which had been fused and cultured in Plastic Film Dishes (PFD’s ref, 3,4) A radiochemical HG-PRT microassay, based on the use of the Parafilm Micro Cuvette (PMC; ref. 3,4) was developed, which permits the quantification of the enzyme activity at the single cell level.
KeywordsCell Strain Plastic Film Single Cell Level Genetic Complementation Normal Amniotic Fluid
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