Electrophoretic Separation of Normal and Mutant Phosphoribosylpyrophosphate Synthetase

  • P. Boer
  • O. Sperling
  • A. de Vries
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 41)


Phosphoribosylpyrophosphate (PRPP) synthetase (E.C. catalyzes the formation of PRPP from ribose-5-phosphate and ATP in the presence of Mg++ and inorganic phosphate. The product, PRPP, is a substrate of the first rate limiting step of the de novo synthesis of purine nucleotides and its availability has been shown to regulate this pathway in human tissue (1). A superactive mutant erythrocyte PRPP synthetase with decreased sensitivity to feedback inhibition has recently been found by us in a gouty family (2,3).


Cellulose Acetate Electrophoretic Separation Liver Extract Purine Nucleotide First Rate 
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    Sperling O., Boer P., Persky-Brosh S., Kanarek E. and De Vries A. Europ. J. Clin. Biol. Res. 17:703, 1972.Google Scholar
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    Sperling O., Persky-Brosh S., Boer P. and De Vries A. Bioch. Medicine, In press, 1973.Google Scholar
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    Fox I.H. and Kelley W.N. J. Biol. Chem. 246:5739, 1971.PubMedGoogle Scholar
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    Sperling O., Boer P., Persky-Brosh S. and De Vries A. FEBS letters, 27:229, 1972.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1974

Authors and Affiliations

  • P. Boer
    • 1
  • O. Sperling
    • 1
  • A. de Vries
    • 1
  1. 1.Rogoff-Wellcome Medical Research Institute and the Metabolic Unit of Department of Medicine DTel-Aviv University Medical School, Beilinson HospitalPetah TikvaIsrael

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