Murine Coronavirus RNA

  • Julian L. Leibowitz
  • Susan R. Weiss
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 142)


Coronaviruses are ubiquitous in nature and cause several diseases in infected hosts (Robb and Bond, 1979a). The genomic RNA of coronaviruses has been extensively studied in recent years (Lomneczi and Kennedy, 1977; Yogo et al., 1977; Lai and Stohlman, 1978; Wege et al., 1978; Macnaughton and Madge, 1978; Guy and Brian, 1979). These studies have shown that the coronavirus genome is a large, single stranded RNA which is polyadenylated and of positive polarity.


Infectious Bronchitis Virus Nucleocapsid Protein Mouse Hepatitis Virus Subgenomic RNAs Tris Acetate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bailey, J.M., and Davidson, N., 1976, Methylinercury as a reversible denaturing agent for agarose gel electrophoresis, Anal. Biochem., 70: 75.PubMedCrossRefGoogle Scholar
  2. Bond, C.W., Leibowitz, J. L, and Robb, J.A., 1979, Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V, Virology, 94: 371.PubMedCrossRefGoogle Scholar
  3. Borun, T.W., Scharff, M.D., and Robbin, E., 1967, Preparation of mammalian polyribosomes with the detergent Nonidat P-40, Biochim. Biophys. Acta, 149: 302.PubMedCrossRefGoogle Scholar
  4. Cleveland, D.W., Fischer, S.G., Kirschner, M.W., and Laemmli, U.K., 1977, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis, J. Biol. Chem., 252: 1102.PubMedGoogle Scholar
  5. Gibson, W., 1974, Polyoma virion proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis, Virology, 62: 319.PubMedCrossRefGoogle Scholar
  6. Guy, J.S., and Brian, D.A., 1979, Bovine coronavirus genome, J. Virol., 29: 293.PubMedGoogle Scholar
  7. Laemmli, U.K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227: 680.PubMedCrossRefGoogle Scholar
  8. Lai, M.M.C., and Stohlman, S.A., 1978, RNA of mouse hepatitis virus, J. Virol., 26: 236.PubMedGoogle Scholar
  9. Laskey, R.A., and Mills, A.D., 1977, Enhanced autoradiographic detection of 32p and 125I using intensifying screens and hypersensitized film. FEBS Letters, 82: 314.PubMedCrossRefGoogle Scholar
  10. Lee, Y.F., Kitamura, N., Nomoto, A., and Wimmer, E., 1979, Sequence studies of poliovirus type 1, type 2, and two type 1 defective interfering particles RNAs1 and fingerprint of the poliovirus type 3 genome, J. Gen. Virol., 44: 311.PubMedCrossRefGoogle Scholar
  11. Lomniczi, B., and Kennedy, I., 1977, Genome of infectious bronchitis virus, J. Virol., 24: 99.PubMedGoogle Scholar
  12. Macnaughton, M.R., and Madge, M.H., 1978, The genome of human coronavirus strain 229E, J. Gen. Virol., 39: 497.PubMedCrossRefGoogle Scholar
  13. McMaster, G.K., and Carmichael, G.C., 1977, Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange, Proc. Natl. Acad. Sci. U.S.A., 75: 4835.CrossRefGoogle Scholar
  14. Pelham, H.R.B., and Jackson, R.J., 1976, An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem., 67: 247.PubMedCrossRefGoogle Scholar
  15. Robb, J.A., and Bond, C.W., 1979a, Coronaviridae, in: “Comprehensive Virology,” vol. 14, H. Fraenkel-Conrat and R.R. Wagner, eds., Plenum Publishing Corp., New York.Google Scholar
  16. Robb, J.A., and Bond, C.W., 1979b, Pathogenic murine coronaviruses. I. Characterization of biological behavior in vitro and virus specific intracellular RNA of strongly neutotropic JHMV and weakly neurotropic A59V viruses, Virology, 94: 352.PubMedCrossRefGoogle Scholar
  17. Siddell, S.G., Wege, H., Barthel, A., and terMeulen, V., 1980, Coronavirus JHM:Cell-free synthesis of structural protein p 60, J. Virol., 33: 10.PubMedGoogle Scholar
  18. Stern, D.F., and. Kennedy, S.I.T., 1980, Coronavirus multiplication strategy. I. Identification and characterization of virusspecified RNA, J. Virol., 34: 665.PubMedGoogle Scholar
  19. Stohlman, S.A., and Lai, M.M.C., 1979, Phosphoproteins of murine hepatitis viruses, Virology, 32: 672.Google Scholar
  20. Sturman, L.S., and Takemoto, K.K., 1972, Enhanced growth of a murine coronavirus in transformed mouse cells. Infection & Immunity, 6: 501.Google Scholar
  21. Wege, H., Muller, A., and terMeulen, V., 1978, Genomic RNA of the murine coronavirus JHM, J. Gen. Virol., 41: 217.PubMedCrossRefGoogle Scholar
  22. Wilt, F.H., 1977, The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggs, Cell, 11: 673.PubMedCrossRefGoogle Scholar
  23. Yogo, Y., Hirano, N., Hino, S., Shibuta, H., and Matumoto, M., 1977, Polyadenylate in the virion RNA of mouse hepatitis virus, J. Biochem., 82: 1103.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Julian L. Leibowitz
    • 1
  • Susan R. Weiss
    • 2
  1. 1.Department of PathologyUniversity of California, San Diego, School of MedicineLa JollaUSA
  2. 2.Department of MicrobiologyUniversity of California, School of MedicineSan FranciscoUSA

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