Cloning and molecular characterization of monoclonal antibody-defined ovarian tumour antigens

  • I. G. Campbell
  • W. D. Foulkes
  • T. A. Jones
  • L. G. Poels
  • J. Trowsdale


In an effort to develop additional treatments and improved methods for early detection of ovarian cancers a number of monoclonal antibodies (MAbs) have been generated against cell surface antigens of epithelial ovarian cancer (EOC). Therapies using these reagents to target ovarian cancers have so far been disappointing. Identifying the antigens recognized by the antibody may be important for improving the targeting of antibody based therapy. For example, detailed molecular analysis of the polymorphic epithelial mucin gene, MUCl, has shown that differential glycosylation of a tandem repeat unit is responsible for the appearance of novel epitopes in breast, ovarian and other adenocarcinomas [1]. This information has opened up new possibilities for immunotherapy with mucins since these structures are highly immunogenic and are relatively tumour specific. The encouraging results of such studies prompted us to undertake the cloning of the genes for other MAb defined tumour antigens based on an expression cloning technique [2]. In this chapter we briefly summarize the important characteristics of the genes cloned so far and discuss their possible function in ovarian cancer.


Ovarian Cancer Epithelial Ovarian Cancer Folate Receptor Immunoglobulin Superfamily Epithelial Ovarian Cancer Patient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Taylor-Papadimitriou, J., Allen, D., Granowska, M. et al, (1992) Molecular structure and clinical applications of a cancer-associated mucin. InOvarian Cancer, 2, Biology, Diagnosis and Management, (eds F. Sharp, W. P. Mason and W. Creasman), Chapman Hall, London, pp. 39–50.Google Scholar
  2. 2.
    Seed, B. (1987) An LFA-3 cDNA encodes a phospholipid membrane protein homologous to its receptor CD2. Nature, 329,840–2.PubMedCrossRefGoogle Scholar
  3. 3.
    Campbell, I.G., Foulkes, W.D., Jones, T.A. et al, (1992) Cloning of genes encoding ovarian carcinoma-specific antigens. In Ovarian Cancer, 2, Biology, Diagnosis and Management, (eds F. Sharp, W.P. Mason and W. Creasman), Chapman & Hall, London, pp. 73–86.Google Scholar
  4. 4.
    Campbell, I.G., Jones, T.A., Foulkes, W.D. et al, (1991) Folate binding protein is a marker for ovarian cancer. Cancer Res., 51,5329–38.PubMedGoogle Scholar
  5. 5.
    Henderson, G.B. (1990) Folate binding proteins. Ann. Rev. Nutr., 10,319–35.CrossRefGoogle Scholar
  6. 6.
    Garin-Chesa, P., Campbell, L, Saigo, P. F. et al., (1993) Trophoblast and ovarian cancer antigen LK26.Am. J. Pathol, 142,557–67.PubMedGoogle Scholar
  7. 7.
    Poels, L.G., Peters, D., van Megen, Y. et al, (1986) Monoclonal antibody against human ovarian tumor associated antigens. J. Natl Cancer Inst., 76, 781–91.PubMedGoogle Scholar
  8. 8.
    Buist, M.R., Kenemans, P., Vermerken, J.B. et al, (1992) Radioimmunotargeting in ovarian carcinoma patients with indium-Ill labelled monoclonal antibody OV-TL3 F(ab02: pharmacokinetics, tissue distribution, tumour imaging. Int. J. Gynecol Cancer, 2,23–34.PubMedCrossRefGoogle Scholar
  9. 9.
    Campbell, LG., Freemont, P.S., Foulkes, W.D. et al, (1992) An ovarian tumour marker with homology to vaccinia virus contains an IgVlike region and multiple transmembrane domains. Cancer Res., 52,5416–20.PubMedGoogle Scholar
  10. 10.
    Williams, A.F. and Barclay, A.N. (1988) The immunoglobulin superfamily — domains for cell surface recognition. Ann. Rev. Immunol, 6, 318–405.CrossRefGoogle Scholar
  11. 11.
    Smith, G.L., Chan, Y.S. and Howard, ST. (1991) Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted 18. terminal repeat. J. Gen. Virol, 72,1349–76.PubMedCrossRefGoogle Scholar
  12. 12.
    Massuger, L.F.A., Kenemans, P., Claessens, R.A.M.J. et al, (1991) Detection and localization of ovarian cancer with radiolabeled monoclonal antibodies. Eur. J., Obstet. Gynecol Reprod. Biol, 41,47–63.PubMedCrossRefGoogle Scholar
  13. 13.
    Boerman, O.C., Makkink, W.K., Thomas, C.M.G. et al, (1990) Monoclonal antibodies that discriminate between human ovarian carcinomas and benign ovarian tumours. Eur. J. Cancer, 26,117–27.PubMedCrossRefGoogle Scholar
  14. 14.
    Simmonds, D., Makgoba, M.W. and Seed, B. (1988) ICAM, an adhesion ligand of LFA-1, is homologous to the neural-cell adhesion molecule NCAM. Nature, 331,624–7.CrossRefGoogle Scholar
  15. 15.
    Johnson, J.P. (1991) Cell adhesion molecules of the immunoglobulin supergene family and their role in malignant transformation and 22. progression to metastatic disease. Cancer Metastasis Rev., 10,11–12.PubMedCrossRefGoogle Scholar
  16. 16.
    Holzmann, B., Brocker, E.B., Lehmann, J.M. et al, (1987) Tumor progression in human malignant melanoma: five stages defined by their 23. antigenic phenotypes. Int. J. Cancer, 39,466–71.PubMedCrossRefGoogle Scholar
  17. 17.
    Favrot, M.C., Combaret, V., Goillot, E. et al, (1991) Expression of leucocyte adhesion molecules on 66 clinical neuroblastoma specimens. Int. J. Cancer, 48,502–10.PubMedCrossRefGoogle Scholar
  18. 18.
    Tsujisaki, M., Imai, K., Hirata, H. et al, (1991) Detection of circulating intercellular adhesion molecule-1 ntigen in malignant diseases. Clin. Exp. Immunol, 85,3–8.PubMedCrossRefGoogle Scholar
  19. 19.
    Rettig, W.J., Chesa, P.C., Beresford, H.R. et al, (1986) Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets.Cancer Res., 46, 6406–12.PubMedGoogle Scholar
  20. 20.
    Rettig, W.J., Garin-Chesa, P., Beresford, H.R. et al, (1988) Cell-surface glycoproteins of human sarcomas: differential expression in normal and malignant tissues and cultured cells. Immunology, 85,3110–14.Google Scholar
  21. 21.
    Foulkes, W.D., Campbell, I.G. Stamp, G.W.H. et al, (1993) Loss of heterozygosity and amplification on chromosome llq in human ovarian cancer. Br. J. Cancer, 67,268–73.PubMedCrossRefGoogle Scholar
  22. 22.
    Miotti, S., Canevari, S., Menard, S. et al, (1987) Characterization of human ovarian carcinoma-associated antigens defined by monoclonal antibodies with tumor-restricted specificity. Int. J. Cancer, 39,297–303.PubMedCrossRefGoogle Scholar
  23. 23.
    Ragoussis, J., Senger, G., Trowsdale, J. et al, (1992) Genomic organisation of the human folate receptor genes on chromosome 11ql3. Genomics, 14,423–30.PubMedCrossRefGoogle Scholar

Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • I. G. Campbell
  • W. D. Foulkes
  • T. A. Jones
  • L. G. Poels
  • J. Trowsdale

There are no affiliations available

Personalised recommendations