Vacuolar Encephalomyelopathy in Mice Induced by Intracerebral Inoculation with a Coronavirus JHM-CC Strain

  • Tetsuro Tsukamoto
  • Yuzo Iwasaki
  • Norio Hirano
  • Satoru Haga
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)


JHM-CC virus, a mutant strain of mouse hepatitis virus, was isolated by Hirano in 1980 from a DBT cell culture persistently infected with the neurovirulent strain, JHM1. This mutant strain is characterized by formation of smaller plaques on the DBT cells and by lower cell virulence than the original JHM1. Although several murine coronaviruses such as JHM are known to cause demyelinating encephalitis2,4, we found that JHM-CC does not induce primary demyelination, but instead produces characteristic vacuolar degeneration in the brain and spinal cord. In this report we describe the clinical manifestations, neuropathological changes and viral antigen distribution in the central nervous system (CNS) of ICR mice infected intracerebrally with JHM-CC.


Viral Antigen Vacuolar Degeneration Mouse Hepatitis Virus Habenular Nucleus Vacuolar Change 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Tetsuro Tsukamoto
    • 1
  • Yuzo Iwasaki
    • 2
  • Norio Hirano
    • 3
  • Satoru Haga
    • 3
  1. 1.Department of NeurologyFukushima Medical CollegeFukushima, SendaiJapan
  2. 2.Department of Neurological SciencesTohoku University School of MedicineSendaiJapan
  3. 3.Department of Veterinary MicrobiologyIwate UniversityMoriokaJapan

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