A Comparison of Bovine Coronavirus Strains Using Monoclonal Antibodies

  • M. A. Clark
  • I. Campbell
  • A. A. El-Ghorr
  • D. R. Snodgrass
  • F. M. M. Scott
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)


Eight monoclonal antibodies (MAbs) were raised against a bovine Coronavirus (B.C.V.) which had been isolated in Scotland and was designated S2. The MAbs were divided into two groups on the basis of their reactions with S2 virus in indirect immunofluorescence (I.F.), neutralisation and haemagglutination inhibition (H.A.I.) tests. Five of the MAbs were positive by all three tests but failed to bind to proteins in Western immunoblotting experiments. The remaining three MAbs were positive in I.F. tests only, two of which were shown to bind to the 52K nucleocapsid protein by Western immunoblotting. Different patterns of antigen distribution within infected cells were demonstrated when the MAbs were used in the I.F. test. However only minor strain variations were detected by I.F. and H.A.I. tests when the MAbs were tested against each of five cell culture adapated strains of B.C.V. Twenty-nine isolates of B.C.V. have been grown in neonatal calf tracheal organ cultures: attempts are being made to further characterise these isolates.


Ascitic Fluid Haemagglutination Inhibition Neonatal Calf Membrane Fluorescence Tracheal Organ Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    B. King and D.A. Brian. Bovine Coronavirus structural proteins. J. Virol. 42:700 (1982).PubMedGoogle Scholar
  2. 2.
    D. Deregt, M. Sahara, and L.A. Babiuk. Structural proteins of bovine Coronavirus and their intracellular processing. J. gen. Virol. 68: 2863 (1987).PubMedCrossRefGoogle Scholar
  3. 3.
    D. Deregt and L.A. Babiuk. Monoclonal antibodies to bovine Coronavirus: Characteristics and topographical mapping of neutralising epitopes on the E2 and E3 glycoproteins. Virology, 161: 410 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    D. Deregt, G.A. Gifford, M.K. Ijaz, T.C. Watts, J.E. Gilchrist, D.M. Haines, and L.A. Babiuk. Monoclonal antibodies to bovine Coronavirus glycoproteins E2 and E3: Demonstration of in vivo virus -neutralizing activity. J. gen. Virol. 70: 993 (1989).PubMedCrossRefGoogle Scholar
  5. 5.
    B. King, B.J. Potts, and D.A. Brian. Bovine Coronavirus haemagglutinin protein. Virus Res. 2: 53 (1985).PubMedCrossRefGoogle Scholar
  6. 6.
    M.D. Parker, G.J. Cox, D. Deregt, D.R. Fitzpatrick, and L.A. Babiuk. Cloning and in vitro expression of the gene for the E3 haemagglutinin glycoprotein of bovine Coronavirus. J. gen. Virol. 70: 155 (1989).PubMedCrossRefGoogle Scholar
  7. 7.
    A.A. El-Ghorr, D.R. Snodgrass, P.M.M. Scott, and I. Campbell. A serological comparison of bovine Coronavirus strains. Arch. Virol. 104: 241 (1989).PubMedCrossRefGoogle Scholar
  8. 8.
    J.F. Vautherot, J. Laporte, M.F. Madelaine, P.Bobulesco, and A. Roseto. Antigenic and polypeptide structure of bovine enteric Coronavirus as defined by monoclonal antibodies. Adv. Exp. Med. Biol. 173: 117 (1984).PubMedGoogle Scholar
  9. 9.
    S. Dea, R.S. Roy, and M.A.S.Y. Elazhary. Antigenic variations among calf diarrhoea coronaviruses by immunodiffusion and counterimmunoelectrophoresis. Ann. Rech. Vet. 13(4): 351 (1982).PubMedGoogle Scholar
  10. 10.
    E.J. Stott, L.H. Thomas, J.C.Bridger, and N.J. Jebbett. Replication of a bovine Coronavirus in organ cultures of foetal trachea. Vet. Microbiol. 1: 65 (1976).CrossRefGoogle Scholar
  11. 11.
    J.C. Bridger, G.N. Woode, and A. Meyling. Isolation of coronaviruses from neonatal calf diarrhoea in Great Britain and Denmark. Vet. Microbiol. 3:101 (1978).CrossRefGoogle Scholar
  12. 12.
    M.S. McNulty, D.G. Bryson, G.M. Allan, and E.F. Logan. Coronavirus infection of the bovine respiratory tract.VetMicrobiol. 9:425 (1984).PubMedCrossRefGoogle Scholar
  13. 13.
    A.A. El-Ghorr, D.R. Snodgrass, and F.M.M. Scott. Evaluation of an immunogold electron microscopy technique for detecting bovine Coronavirus. J. Virol. Methods. 19:215 (1988).PubMedCrossRefGoogle Scholar
  14. 14.
    C.A. Mebus, E.L. Stair, M.B. Rhodes, and M.J. Twiehaus. Neonatal calf diarrhoea: Propagation, attenuation, and characteristics of a coronavirus-like agent. Am. J. Vet. Res. 34:145 (1973).PubMedGoogle Scholar
  15. 15.
    S. Dea, R.S. Roy, and M.E. Begin. Physicochemical and biological properties of neonatal calf diarrhoea coronaviruses isolated in Quebec and comparison with the Nebraska calf Coronavirus. Am. J. Vet. Res. 41:23 (1980).PubMedGoogle Scholar
  16. 16.
    D.J. Reynolds, T.G. Debney, G.A. Hall, L.H. Thomas, and K.R. Parsons. Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Arch. Virol. 85: 71 (1985).PubMedCrossRefGoogle Scholar
  17. 17.
    L.H. Thomas and C.J. Howard. Effect of mycoplasma dispar, mycoplasma bovirhinis, acholeplasma laidlawii and T-mycoplasmas on explant cultures of bovine trachea. J. Comp. Path. 84: 193 (1974).PubMedCrossRefGoogle Scholar
  18. 18.
    A.R. Collins, R.L. Knobler, H. Powell, and M.J. Buchmeier. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion. Virology, 119:358 (1982).PubMedCrossRefGoogle Scholar
  19. 19.
    H. Laude, J-M. Chapsal, J. Gelfi, S. Labiau, and J. Grosclaude. Antigenic structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins. J. gen. Virol. 67: 119 (1986).Google Scholar
  20. 20.
    S. Dea and P. Tijssen. Antigenic and polypeptide structure of turkey enteric coronaviruses as defined by monoclonal antibodies. J. gen. Virol. 70: 1725 (1989).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • M. A. Clark
    • 1
  • I. Campbell
    • 1
  • A. A. El-Ghorr
    • 1
  • D. R. Snodgrass
    • 1
  • F. M. M. Scott
    • 1
  1. 1.Moredun Research InstituteEdinburghUK

Personalised recommendations