Characterization and Location of the Structural Polypeptides of Turkey Enteric Coronavirus Using Monoclonal Antibodies and Enzymatic Treatments

  • Serge Dea
  • Simon Garzon
  • Peter Tijssen
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)


Turkey enteric Coronavirus (TCV) is one of the major causes of epidemic diarrhoea in turkey poults (1, 2). The morphological and physicochemical characteristics of TCV resemble those of other members of the family Coronaviridae (3, 4). However, little is known with respect to the molecular and antigenic structure of the TCV virion, due to difficulties in propagating TCV strains in tissue cultures and lack of highly specific immunological probes (1, 4). Field isolates can be propagated by oral inoculation and intestinal infections of young turkey poults, or by inoculation into embryonating turkey eggs (4, 5). TCV possesses a hemagglutinating (HA) activity which may be associated to short granular projections located near the base of the characteristic larger bulbous peplomers (6). Recently, we adapted TCV isolates in HRT-18 cells, an established cell line derived from human rectum adenocarcinoma (7). In these cells, TCV induces cytopathic changes, including polykaryocytosis, which depended on trypsin in the culture medium (8).


Infectious Bronchitis Virus Western Immunoblotting Infectious Bronchitis Virus Strain Polypeptide Structure Turkey Poult 
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Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Serge Dea
    • 1
  • Simon Garzon
    • 1
  • Peter Tijssen
    • 1
  1. 1.CRMC, Institute Armand-FrappierUniversity of QuebecLaval-des-RapidesCanada

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