Binding of Antibodies that Strongly Neutralise Infectious Bronchitis Virus is Dependent on the Glycosylation of the Viral Peplomer Protein

  • G. Koch
  • A. Kant
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 276)


Most of the biological properties of infectious bronchitis virus (IBV) are associated with the S1 glycoprotein (1) that together with the S2 glycoprotein forms the peplomer protein (2,3). Both glycoproteins bear N-linked oligosaccharides (4).


  1. 1.
    D. Cavanagh and P.J. Davis, Coronavirus IBV: Virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection, J. Gen. Virol. 67: 1435–1442 (1986).PubMedCrossRefGoogle Scholar
  2. 2.
    D.F. Stern, L. Burgess, and B.M. Sefton, Structural analysis of virion proteins of the Avian Infectious Bronchitis Virus, J. Virology 42: 208–219 (1982).PubMedGoogle Scholar
  3. 3.
    D. Cavanagh, Coronavirus IBV: structural characterization of the spike protein, J. Gen. Virol. 64: 2577–2583 (1983).PubMedCrossRefGoogle Scholar
  4. 4.
    D.F. Stern and B.M. Sefton, Coronavirus proteins: Structure and function of the oligosaccharides of the Avian Infectious Bronchitis Virus glycoproteins, J. Virology 42: 804–812 (1982).Google Scholar
  5. 5.
    G. Koch, L. Hartog, A. Kant, D. van Roozelaar, and G.F. de Boer, Differentiation of IBV variant strains employing monoclonal antibodies, Isr. J. Vet. Med. 42: 89–97 (1986).Google Scholar
  6. 6.
    H. Towbin, T. Staehlin, and J. Gordon, Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets. Procedure and some applications, Proc. Natl. Acad. Sci. 6: 4350–4354 (1979).CrossRefGoogle Scholar
  7. 7.
    R. Hawkes, Identification of Concanavalin A-binding proteins after sodiumdodecyl sulfate-gel electrophoresis and protein blotting, Anal. Biochem. 123: 143–146 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    J.A. Lenstra, J.G. Kusters, G. Koch, and B.A.M. van der Zeijst, Antigenicity of the peplomer protein of infectious bronchitis virus, Mol. Immunol. 1: 7–15 (1989).CrossRefGoogle Scholar
  9. 9.
    J.G. Kusters, E.J. Jager, J.A. Lenstra, G. Koch, W.P.A. Posthumus, R.H. Meloen, and B.A.M. van der Zeijst, Analysis of an immunodominant region of avian Coronavirus IBV, J. Immunol., in press, (1989).Google Scholar
  10. 10.
    D. Cavanagh, Coronavirus IBV glycopeptides size of the polypetide moieties and nature of their oligosaccharides, J. Gen. Virol. 64: 1187–1191 (1983).PubMedCrossRefGoogle Scholar
  11. 11.
    M.M. Binns, M.E.G. Boursnell, D. Cavanagh, D.J.C. Pappin, and T.D.K. Brown, Cloning and sequencing of the gene encoding the spike protein of the Coronavirus IBV, J. Gen. Virol., 66: 719–726 (1985).PubMedCrossRefGoogle Scholar
  12. 12.
    Tarentino, A.L., C.M. Gómes, and T.H. Plummer, Deglycosylation of aspargine-linked glycans by peptide:N-glycosidase F, Biochem. 24: 4665–4671 (1985).CrossRefGoogle Scholar
  13. 13.
    J.H. Elder, and S. Alexander, Endo-β-acetylglucosaminidase F: Endo -glycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins, Biochem. 79: 4550–4544 (1982).Google Scholar
  14. 14.
    A.L. Tarentino, T.H. Plummer, and F. Maley, Purification and properties of an endo-N-acetylglucosaminidase H from Streptomyces griseus ,J. Biol. Chem. 249: 811-–17 (1974).PubMedGoogle Scholar
  15. 15.
    Rose, M.C., W.A. Voter, H. Sage, C.F. Brown, B. Kaufman, S. Basu, and B. Bartholomew, J. Biol. Chem. 259: 3167–3172 (1984).PubMedGoogle Scholar
  16. 16.
    H.M. Geysen, S.J. Rodda, and T.J.Mason, The delineation of peptides able to mimic assembled epitopes, In:Synthetic peptides as antigens Ciba Found. Symp. 119, J. Wiley & Sons, Chichester (1986).Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • G. Koch
    • 1
  • A. Kant
    • 1
  1. 1.Dept. of VirologyCentral Veterinary InstituteLelystadThe Netherlands

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