Sequence Analysis of the 3′ End (8740 Nucleotides) of BECV Genome; Comparison with Homologous MHV Nucleotide Sequence
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Bovine enteritic Coronavirus (BECV) is one of the major pathogen of neonatal calves. The viral genome is a unique 20 Kb large RNA molecule which is polyadenylated at its 3′ end. Studies of viral mRNAs suggest that eight genes are translated (Crucière & Laporte, personnal communication) and as established for MHV1 the possibility of functional bicistronic genes cannot be excluded. BECV is made of 4 major structural proteins2,3,4, a 50 kD phosphorylated nucleocapsid N, a 180 kD peplomer glycoprotein S (present on the virions as 105 and 95 kD glycosylated subunits), a 125 kD haemagglutinin HE (made up of two disulfide bridge-linked glycosylated subunits of 65 kD) and a 28 kD transmembrane glycoprotein M. The complete amino-acid sequences of these proteins have recently been deduced from nucleotide sequences of the viral genome5,6,7,8,9.
KeywordsRelative Molecular Mass Mouse Hepatitis Virus Neonatal Calf Bovine Coronavirus Consensus Nucleotide Sequence
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