On the Membrane Cytopathology of Mouse Hepatitis Virus Infection as Probed by a Semi-Permeable Translation-Inhibiting Drug
- 322 Downloads
Previous studies of the membrane fusion process have permitted the characterization of membrane permeability changes concomitant with MHV-induced cytopathology. One indication of membrane permeability in MHV-infected cells is their sensitivity to translational inhibition by the normally impermeable amino-glycoside, hygromycin B (Macintyre, G., Wong, F. and Anderson, R. (1989) J. Gen. Virol. 70, 763–768). In the present study, we examine the hygromycin B sensitivity of acutely infected mouse fibroblast L-2 cell and macrophage cultures as well as persistently infected mouse fibroblast LM-K cell cultures. The results suggest that membrane permeability alterations (as indicated by hygromycin B sensitivity) are a common feature of these MHV infections. Hygromycin B “cured” persistently infected LM-K cells as indicated by the absence of detectable virus antigen by immunofluorescence and by the absence of infectious virus even after removal of the drug or co-cultivation with untreated L-2 cells. The results argue against the maintenance of MHV infection by a mechanism involving latently or non-cytolytically infected cells. We conclude therefore that at least one mechanism for MHV persistence depends on virus propagation by cytolytic infection of a small, dynamically changing, fraction of the total cells present in culture.
KeywordsMinimal Essential Medium Peritoneal Macrophage Viral Antigen Infectious Virus Demyelinating Disease
- 6.Lucas, A., Coulter, M., Anderson, R., Dales, S. and Flintoff, W. (1978) In vivo and in vitro models of demyelinating diseases. II. Persistence and host-regulated thermosensitivity in cells of neural derivation infected with mouse hepatitis and measles viruses. Virology 88:325–337.PubMedCrossRefGoogle Scholar
- 13.Sorensen, O., Beushausen, S., Puchalski, S., Cheley, S., Anderson, R., Coulter-Mackie, M. and Dales, S. (1984) In vitro and in vivo models of demyelinating diseases -VIII: genetic, immunologic and cellular influences on JHM virus infection of rats. Adv. Exp. Med. Biol. 173:279–298.PubMedGoogle Scholar
- 16.Rothfels, K.H., Axelrad, A.A., Siminovitch, L, McCulloch, E.A. and Parker, R.C. (1959) The origin of altered cell lines from mouse, monkey and man as indicated by chromosome and transplantation studies. Can. Cancer Conf. 3:189–214.Google Scholar
- 21.Cameron, J.M., Clemens, M.J., Gray, M.A., Menzies, D.E., Mills, B.J., Warren, A.P. and Pasternak, C.A. (1986) Increased sensitivity of virus-infected cells to inhibitors of protein synthesis does not correlate with changes in plasma membrane permeability. Virology 155:534–544.PubMedCrossRefGoogle Scholar