Determination of Intracellular pH by Color Film Histophotometry of Frozen in Situ Rat Brain

  • Joseph C. LaManna
  • Kimberly A. McCracken
  • Tim S. Whittingham
  • W. David Lust
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 200)


Regulation of intracellular pH (pHi) is a critical factor in brain function in normal and pathological states. An inability to maintain controlled pHi during and after conditions which impose severe metabolic stress on the brain has been implicated as contributory to permanent neurologic damage in severe hypoxia, ischemic stroke, and seizures (1,2). The relationship between energy metabolism and pHi has been difficult to study because of spatial and temporal heterogeneity in brain physiology. Furthermore, there is a lack of effective techniques for determining pHi in brain which are compatible with concurrent measurement, or control, of other variables which affect or reflect cell physiology. For example, tissue lactate levels have been considered to reflect pHi, but this relationship has not been verified by direct experimental determination. The quantitative method for determining tissue lactate, which also preserves spatial distribution, excludes most existing techniques for quantitative pH measurement. This problem might be resolved by the use of colorimetric pH indicator dyes. The requirements for an in vivo colorimetric pH indicator in the mammalian central nervous system are stringent, but can be met by the vital dye neutral red (3).


Middle Cerebral Artery Occlusion Color Slide Tissue Lactate Permanent Neurologic Damage Wavelength Absorption Peak 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Joseph C. LaManna
    • 1
    • 3
  • Kimberly A. McCracken
    • 1
    • 3
  • Tim S. Whittingham
    • 2
  • W. David Lust
    • 2
  1. 1.Department of NeurologyUniversity Hospitals of ClevelandClevelandUSA
  2. 2.Division of NeurosurgeryUniversity Hospitals of ClevelandClevelandUSA
  3. 3.Departments of Neurology and PhysiologyCase Western Reserve University School of MedicineClevelandUSA

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