Regional Quantitative Biochemistry and Autoradiography of Protein Synthesis and Serum Extravasation in Brain Edema
Since cerebral metabolic processes are mediated and controlled by enzymes and regulating peptides such as neurohormones, there is considerable interest to relate altered synthesis rates of single specific proteins to the functional activity in various cerebral structures, especially in altered physiological states. The basic mechanisms underlying changes in protein synthesis can be analyzed on a regional level by investigation of the total cellular protein biosynthesis in vivo by radioactive amino acid incorporation2. Coronal sections subjected to autoradiography for regional visualization of in vivo labeled proteins may be further utilized for demonstration of single specific proteins by direct localization of their immunoreactive sites with radioactively labeled antibodies. A correlation can also be carried out with regional blood flow and the energy state of the brain, using appropriate autoradiograph-ic10,9, bioluminescent6,8 and fluoroscopic11 techniques.
KeywordsCold Injury Amino Acid Incorporation Vasogenic Brain Edema Radioactive Amino Acid Cold Lesion
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- 2.Bodsch W, Hossmann KA: Regional protein and RNA synthesis in rat brain. Autoradiography and quantitative HPLC. Neurosci Lett Suppl 7: 431 (1981).Google Scholar
- 3.Bodsch W, Hossmann KA: A quantitative regional analysis of amino acids involved in rat brain protein synthesis by high performance liquid chromatography. J Neurochem, in press (1982).Google Scholar
- 4.Bodsch W, Hürter T, Hossmann KA: Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of the rat brain. Brain Res, in press (1982).Google Scholar
- 5.Klatzo I, Chui E, Fujiwara K, Spatz M: Resolution of vasogenic brain edema. Adv Neurol 28: 359–373 (1980).Google Scholar
- 9.Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L: Measurement of local cerebral blood flow with iodo (14C) antipyrine. Am J Physiol 234: H59–66 (1978).Google Scholar
- 10.Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M: The (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28: 897–916 (1977).CrossRefGoogle Scholar