Regional Quantitative Biochemistry and Autoradiography of Protein Synthesis and Serum Extravasation in Brain Edema

  • Wolfram Bodsch
  • Giinter Mies
  • Wulf Paschen
  • Konstantin-Alexander Hossmann


Since cerebral metabolic processes are mediated and controlled by enzymes and regulating peptides such as neurohormones, there is considerable interest to relate altered synthesis rates of single specific proteins to the functional activity in various cerebral structures, especially in altered physiological states. The basic mechanisms underlying changes in protein synthesis can be analyzed on a regional level by investigation of the total cellular protein biosynthesis in vivo by radioactive amino acid incorporation2. Coronal sections subjected to autoradiography for regional visualization of in vivo labeled proteins may be further utilized for demonstration of single specific proteins by direct localization of their immunoreactive sites with radioactively labeled antibodies. A correlation can also be carried out with regional blood flow and the energy state of the brain, using appropriate autoradiograph-ic10,9, bioluminescent6,8 and fluoroscopic11 techniques.


Cold Injury Amino Acid Incorporation Vasogenic Brain Edema Radioactive Amino Acid Cold Lesion 
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  1. 1.
    Baker RN, Cancilla PA, Pollock PS, Frommes SP: The movement of exogenous protein in experimental edema. An electronmicroscopic study after freeze injury. J Neuropathol Exp Neurol 30: 668–679 (1971).CrossRefGoogle Scholar
  2. 2.
    Bodsch W, Hossmann KA: Regional protein and RNA synthesis in rat brain. Autoradiography and quantitative HPLC. Neurosci Lett Suppl 7: 431 (1981).Google Scholar
  3. 3.
    Bodsch W, Hossmann KA: A quantitative regional analysis of amino acids involved in rat brain protein synthesis by high performance liquid chromatography. J Neurochem, in press (1982).Google Scholar
  4. 4.
    Bodsch W, Hürter T, Hossmann KA: Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of the rat brain. Brain Res, in press (1982).Google Scholar
  5. 5.
    Klatzo I, Chui E, Fujiwara K, Spatz M: Resolution of vasogenic brain edema. Adv Neurol 28: 359–373 (1980).Google Scholar
  6. 6.
    Kogure K, Alonso OF: A pictorial representation of endogenous brain aTP by a bioluminescent method. Brain Res 154: 273–284 (1978).CrossRefGoogle Scholar
  7. 7.
    Mies G, Niebuhr I, Hossmann KA: Simultaneous measurement of blood flow and glucose metabolism by autoradiographic techniques. Stroke 12: 581–588 (1981).CrossRefGoogle Scholar
  8. 8.
    Paschen W, Niebuhr I, Hossmann KA: A bioluminescence method for the demonstration of regional glucose distribution in brain slices. J Neurochem 36: 513–517 (1981).CrossRefGoogle Scholar
  9. 9.
    Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L: Measurement of local cerebral blood flow with iodo (14C) antipyrine. Am J Physiol 234: H59–66 (1978).Google Scholar
  10. 10.
    Sokoloff L, Reivich M, Kennedy C, Des Rosiers MH, Patlak CS, Pettigrew KD, Sakurada O, Shinohara M: The (14C) deoxyglucose method for the measurement of local cerebral glucose utilization: Theory, procedure, and normal values in the conscious and anesthetized albino rat. J Neurochem 28: 897–916 (1977).CrossRefGoogle Scholar
  11. 11.
    Welsh FA, Rieder W: Evaluation of in situ freezing of cat brain by NADH fluorescence. J Neurochem 31: 299–309 (1978).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Wolfram Bodsch
    • 1
  • Giinter Mies
    • 1
  • Wulf Paschen
    • 1
  • Konstantin-Alexander Hossmann
    • 1
  1. 1.Max-Planck-Institut für Neurologische ForschungCologneGermany

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