Regional Quantitative Biochemistry and Autoradiography of Protein Synthesis and Serum Extravasation in Brain Edema

  • Wolfram Bodsch
  • Giinter Mies
  • Wulf Paschen
  • Konstantin-Alexander Hossmann

Abstract

Since cerebral metabolic processes are mediated and controlled by enzymes and regulating peptides such as neurohormones, there is considerable interest to relate altered synthesis rates of single specific proteins to the functional activity in various cerebral structures, especially in altered physiological states. The basic mechanisms underlying changes in protein synthesis can be analyzed on a regional level by investigation of the total cellular protein biosynthesis in vivo by radioactive amino acid incorporation2. Coronal sections subjected to autoradiography for regional visualization of in vivo labeled proteins may be further utilized for demonstration of single specific proteins by direct localization of their immunoreactive sites with radioactively labeled antibodies. A correlation can also be carried out with regional blood flow and the energy state of the brain, using appropriate autoradiograph-ic10,9, bioluminescent6,8 and fluoroscopic11 techniques.

Keywords

Cold Injury Amino Acid Incorporation Vasogenic Brain Edema Radioactive Amino Acid Cold Lesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Wolfram Bodsch
    • 1
  • Giinter Mies
    • 1
  • Wulf Paschen
    • 1
  • Konstantin-Alexander Hossmann
    • 1
  1. 1.Max-Planck-Institut für Neurologische ForschungCologneGermany

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