Cell Hybridization: A Tool for the Study of Cell Differentiation
One of the many uses of somatic cell hybridization has been to explore the kinds of regulatory mechanisms responsible for the acquisition and the maintenance of the differentiated state. When this work was begun in the late sixties, it was believed that the genetic analysis of cell differentiation, achieved by analyzing the properties of hybrid cells resulting from the fusion of various kinds of differentiated cells, would make it possible to deduce the kinds of genetic mechanisms responsible for the expression of tissue-specific genes. Since that time, it has become clear that regulation in mammalian cells is far too complex to be dissected by this kind of genetic approach alone. Nevertheless, the results obtained do make it possible to eliminate some kinds of models of cell differentiation. In addition, these studies have revealed that fundamentally different mechanisms are involved in the heritable potential of cells to express tissue specific genes, and in the actual expression of these genes.
Unable to display preview. Download preview PDF.
- Davis, F.M. and Adelberg, A., 1973, Use of somatic cell hybrids for analysis of the differentiated state. Bacteriol. Rev. 27:197–214.Google Scholar
- Ephrussi, B., 1972, “Hybridization of somatic cells”. Princeton University Press, Princeton, NJ.Google Scholar
- Mevel-Ninio, M. and Weiss, M.C., 1981, Immunofluorescent analysis of the time course of extinction, reexpression and activation of albumin production in heterokaryons and hybrids of rat hepatoma cells with mouse fibroblasts. J. Cell Biol., in press.Google Scholar
- Ringertz, N. and Savage, R.E., 1976, “Cell hybrids”. Academic Press, New York.Google Scholar
- Weiss, M.C., 1977, The use of somatic cell hybridization to probe the mechanisms which maintain cell differentiation. In “Human Genetics”, A. Armendares and R. Liskey, eds., Excerpta Medica, Amsterdam, 284–292.Google Scholar