Regulatory Mechanisms of Synaptic Transmission pp 241-260 | Cite as
Brain Clathrin: A Study of Its Properties
Abstract
In 1968, Puszkin, Berl and coworkers reported the presence of contractile proteins in whole brain (1). Subsequent work showed the presence of contractile proteins in nerve-endings (2,3,4). The properties of various contractile proteins found in brain tissue resembled, to a large extent, those from muscle tissue (2,4). As a result, it was proposed that contractile activities are involved in neurotransmitter release (5). With the advantage of sodium dodecyl sulfate polyacrylamide gel electrophoresis as a procedure for distinguishing and comparing the size of various proteins, many researchers working with other tissues or cells began to report the presence and involvement of contractile proteins in motility-related cell functions (6).
Keywords
Synaptic Vesicle DEAE Cellulose Contractile Protein Actin Stress Fiber Coated VesiclePreview
Unable to display preview. Download preview PDF.
References
- 1.Puszkin, S., Berl, S., Puszkin, E. and Clarke, D.D., Actomyosinlike proteins isolated from mammalian brain, Science, 161 (1968) 170–171.PubMedCrossRefGoogle Scholar
- 2.Berl, S. and Puszkin, S., Mg2+-Ca2+-activated adenosine triphosphatase system isolated from mammalian brain, Biochemistry, 9 (1970) 2085–2096.CrossRefGoogle Scholar
- 3.Puszkin, S., Nicklas, W.J. and Berl, S. Actomyosin-like proteins in brain: subcellular distribution, J. Neurochem., 19 (1972) 1319–1330.PubMedCrossRefGoogle Scholar
- 4.Puszkin, S. and Berl, S., Actomyosin-like proteins from brain: separation and characterization of the actin-like component, Biochim. Biophys. Acta, 256 (1972) 695–707.PubMedCrossRefGoogle Scholar
- 5.Berl, S., Puszkin, S. and Nicklas, W.J., Actomyosin-like proteins in brain, Science, 179 (1973) 441–448.PubMedCrossRefGoogle Scholar
- 6.Goldman, R. and Knipe, D., Functions of cytoplasmic fibers in non-muscle cell motility, Cold Spring Harbor Symp. Quant. Biol., 37 (1973) 523–534.CrossRefGoogle Scholar
- 7.Puszkin, S. and Kochwa, S., Regulation of neurotransmitter release by a complex of actin with relaxing protein isolated from rat brain synaptosomes, J. Biol. Chem., 249 (1974) 7711–7715.PubMedGoogle Scholar
- 8.Heuser, J.E. and Reese, T.S., Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction, J. Cell. Biol., 57 (1973) 315–344.PubMedCrossRefGoogle Scholar
- 9.Pearse, B.M.F., Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles, Proc. Natl. Acad. Sci USA, 73 (1976) 1255–1259.PubMedCrossRefGoogle Scholar
- 10.Schook, W. Bloom, W.S., Ores, C., Kochwa, S. and Puszkin S., Mechanochemical properties of brain clathrin: interaction with actin and d-actinin and polymerization into basket-like structures or filaments, Proc. Natl. Acad. Sci. USA, 76 (1979) 116–120.PubMedCrossRefGoogle Scholar
- 11.Puszkin, S., Maimon, J. and Schook, W., Clathrin association with low molecular-weight proteins acting as cofactors for the assembly/disassembly of baskets, J. Cell Biol., 83 (1979) 293a.Google Scholar
- 12.Heuser, J.E. and Reese, T.S., Redistribution of intramembraneous particles from synaptic vesicles: direct evidence for vesicle recycling, Anat. Rec., 181 (1975) 374–384.Google Scholar
- 13.Heuser, J.E., Reese, T.S., Dennis, M.J., Jan, Y. and Evans, L., Synaptic vesicle exocytosis, J. Cell. Biol., 57 (1973) 499–524.CrossRefGoogle Scholar
- 14.Roth, T.F. and Porter, K.R., Yolk protein uptake in the oocyte of the mosquito Aedes aegypti L., J. Cell Biol., 20 (1964) 313–332.PubMedCrossRefGoogle Scholar
- 15.Pearse, B.M.F., Coated vesicles from pig brain, purification and biochemical characterization, J. Mol Biol., 97 (1975), 93–98.PubMedCrossRefGoogle Scholar
- 16.Kanaseki, T. and Kadota, L., The vesicle in a basket: a morphological study of the coated vesicle isolated from the nerve-endings of the guinea pig brain with special reference to the mechanism of membrane movements, J. Cell Biol., 42 (1969) 202–220.PubMedCrossRefGoogle Scholar
- 17.Woodward, M. and Roth, T.F., Influence of buffer ions and divalent cations on coated vesicle disassembly and reassembly, J. Supramolec. Struct., 11 (1979) 237–250.CrossRefGoogle Scholar
- 18.Fine, R.E. and Blitz, A.L., Tropomyosin in brain and growing neurons, Nature, New Biol., 245 (1973) 182–185.CrossRefGoogle Scholar
- 19.Bloom, W.S., Schook, W., Ores, C., Feageson, E. and Puszkin, S., Brain clathrin: viscometric and turbidimetric properties of its ultrastructural assemblies, Biochem. Biophys. Acta, in press.Google Scholar
- 20.Cohen, I. and Cohen, C., A tropomyosin-like protein from human platelets, J. Mol. Biol., 68 (1972) 383–387.PubMedCrossRefGoogle Scholar
- 21.Schook, W., Ores, C. and Puszkin, S., Isolation and properties of brain a-actinin, Biochem. J., 175 (1978) 63–72.PubMedGoogle Scholar
- 22.Puszkin, S., Puszkin, E., Maimon, J., Rouault, C., Schook, W., Ores, C., Kochwa, S. and Rosenfield, R., α-actinin and tropomyosin interactions with a hybrid complex of erythrocyteactin and muscle myosin, J. Biol. Chem., 252 (1977) 5529–5537.PubMedGoogle Scholar
- 23.Gerday, C., Robyns, E., Gosselin-Rey, C., High resolution techniques of peptide mapping: separation of bovine carotid actin peptides on cellulose thin layers and of the corresponding dansylpeptides on polyamide thin layers, J. Chromatog., 38 (1968) 408–411.CrossRefGoogle Scholar
- 24.Lisanti, M., Schook, W., Beckenstein, K, and Puszkin, S., Brain clathrin ultrastructural assemblies: effects of neuroactive compounds and cytoskeletal disruptors, J. Neurochem., (submitted).Google Scholar