Transport of Amino Acids and Catecholamines in Relation to Metabolism and Transmission

  • J. S. de Belleroche
  • H. F. Bradford
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 69)


Synaptosomal preparations from many regions of the CNS provide an excellent experimental opportunity for studying the mechanisms of release and of reuptake of neurotransmitters which are occurring in the intact nervous system. The metabolic performance of synaptosomes and their response to depolarizing stimuli indicates that they are sealed, cell-like, structures, carrying a membrane potential and performing as isolated, working, nerve-terminals3, 12, 13.


Amino Acid Analogue Amino Acid Release Metabolic Performance Synaptosomal Preparation Dopamine Efflux 
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  1. 1.
    de Belleroche, J.S,, and Bradford, H.F., Metabolism of beds of mammalian cortical synaptosomes: response to depolarizing influences. J. Neurochem., 19 (1972) 585–602.CrossRefGoogle Scholar
  2. 2.
    de Belleroche, J.S., and Bradford, H„F„, The stimulus-induced release of acetylcholine from synaptosome beds and its calcium dependence, J, Neurochem., 19 (1972) 1817–1819.CrossRefGoogle Scholar
  3. 3.
    de Belleroche, J.S.,.and Bradford, H.F., The synaptosome: An isolated working neuronal compartment. In G.A. Kerkut and J.W. Phillis (Eds.) Progress In Neurobiology, Vol. 1, Pergamon Press, Oxford (1973) pp. 277–298.Google Scholar
  4. 4.
    de Belleroche, J.S., Bradford, H.S., and Jones, D.A., A study of the metabolism and release of dopamine and amino acids from nerve endings isolated from sheep corpus striatum, J. Neurochem., (1975) in press.Google Scholar
  5. 5.
    de Belleroche, J.S., Dykes, C.R., and Thomas, A.J., The automated separation and analysis of dopamine, its amino acid precursors and metabolites and the application of the method to the measurement of specific radioactivities of dopamine in striated synapto- somes. Analytical Biochem., (1975) in press.Google Scholar
  6. 6.
    Bennett, J.P. Jr., Logan, W.J., and Snyder, S.H., Amino acid neurotransmitter candidates: sodium-dependent high affinity uptake by unique synaptosome fractions, Science, 178 (1972) 997–999.ADSCrossRefGoogle Scholar
  7. 7.
    Bennett, J.P., Logan, W.J., and Snyder, S.H., Amino acids as central nervous transmitters: The influence of ions, amino acid analogues, and ontogeny on transport systems for L-glutamic and L-aspartic acids and glycine into central nervous synaptosomes of the rat, J. Neurochem., 21 (1973) 1533–1550.CrossRefGoogle Scholar
  8. 8.
    Blaustein, M.P., and Goldring, J.M., Membrane potentials in pinched-off presynaptic nerve terminals monitored with a fluorescent probe: evidence that synaptosomes have potassium diffusion potentials, J. Physiol., 247 (1975) 589–615.CrossRefGoogle Scholar
  9. 9.
    Blaustein, M.P., Johnson, E.M., and Needleman, P., Calcium- dependent norepinephrine release from presynaptic nerve-endings in vitro, Proc. Nat. Acad. Sei. U.S.A., 69 (1972) 2237–2240.ADSCrossRefGoogle Scholar
  10. 10.
    Bogdanski, D.F., Tissari, A., and Brodie, B.B., The role of sodium, potassium, ouabain and reserpine in uptake, storage and metabolism of biogenic amines in synaptosomes. Life Sei., 1 (1968) 419–428.CrossRefGoogle Scholar
  11. 11.
    Bradford, H.F., Membrane potentials and metabolic performance in mammalian synaptosomes. In P.F. Benson (Ed.), Cellular Organelles and Membranes in Mental Retardation, Churchill Livingstone, Edinburgh (1971) pp. 1–11.Google Scholar
  12. 12.
    Bradford, H.F., Synaptic preparations for studying neurotransmission at the biochemical level, Biochem. Soc. Trans. 2 (1974)Google Scholar
  13. 13.
    Bradford, H.F., Isolated nerve terminals as an in vitro preparation for the study of dynamic aspects of transmitter metabolism and release. In L.L. Iversen, S.D. Iversen and S.H. Snyder (Eds.) Handbook of Psychopharmacology vol. 1. Plenum Publishing Corporation, New York (1975) pp. 191–252.Google Scholar
  14. 14.
    Carlsson, A., and Linqvist, M., Effect of chlorpromazine or haloperidol on formation of 3-methoxytyramine and normetanephine in mouse brain, Acta Pharmacol, and Toxicol. 20 (1963) 140–144.CrossRefGoogle Scholar
  15. 15.
    Coyle, J.T., and Snyder SH., Catecholamine uptake by synapto- somes in homogenates of rat brain: stereospecificity in different areas, J. Pharmacol. Exp. Ther., 170 (1969) 221–231.PubMedGoogle Scholar
  16. 16.
    Diamond, I., and Fishman, R.A., Development of glucose oxidation in isolated nerve endings, Nature, 243 (1973) 519–520.ADSCrossRefGoogle Scholar
  17. 17.
    Heaton, G.M., and Bachelard, H.S., Fluid spaces of synaptosome beds, J. Neurochem. 22 (1974) 561–564.CrossRefGoogle Scholar
  18. 18.
    Holtz, R.A., and Coyle, J.T., The effects of various salts, temperature and the alkaloids veratridine and batrachotoxin on the uptake of [3H] dopamine into synaptosomes from rat striatum, Mol. Pharmacol., 10 (1974) 746–758.Google Scholar
  19. 19.
    Levi, G., and Raiteri, M., Exchange of neurotransmitter amino acid at nerve endings can stimulate high affinity uptake, Nature, 250 (1974) 735–737.ADSCrossRefGoogle Scholar
  20. 20.
    Marchbanks, R.M., Exchangeability of radioactive acetylcholine with the bound acetylcholine of synaptosomes and synaptic vesicles, Biochem. J., 106 (1968) 87–95.CrossRefGoogle Scholar
  21. 21.
    Martin, D.L., and Smith, A.A., Ions and the transport of butyric acid by synaptosomes, J. Neurochem., 19 (1972) 841–855.CrossRefGoogle Scholar
  22. 22.
    Neal, M.J., and Iversen, L.L., Subcellular distribution of endogenous and -aminobutyric acid in rat cerebral cortex, J. Neurochem. 16 (1969) 1245–1252.CrossRefGoogle Scholar
  23. 23.
    Osborne, R.H., and Bradford, H.F., The influence of eodium, potassium and lanthanum on amino acid release from spinalmedullary synaptosomes, J. Neurochem., 25 (1975) 35–41.CrossRefGoogle Scholar
  24. 24.
    Osborne, R.H., Bradford, H.F., and Jones, D.G., Patterns of amino acid release from nerveendings isolated from spinal cord and medulla, J. Neurochem., 21 (1975) 407–419.CrossRefGoogle Scholar
  25. 25.
    Patrick, R.L., and Barchas, J.D., Regulation of catecholamine synthesis in rat brain synaptosomes, J. Neurochem., 23 (1974) 7–15.CrossRefGoogle Scholar
  26. 26.
    Prakash, N.J., Fontana, J., and Henkin, R.I., Effect of transitional metal ions on (Na+-K+)ATPase activity and the uptake of norepinephrine and choline by rat brain synaptosomes, Life Sciences, 12 (1973) 249–259.CrossRefGoogle Scholar
  27. 27.
    Raiteri, M., Federico, R., Coletti, A., and Levi, G., Release and exchange studies relating to the synaptosomal uptake of GABA, J. Neurochem., 24 (1975) 1243–1250.CrossRefGoogle Scholar
  28. 28.
    Randrup, A., and Munkvad, I., Special antagonism of amphetamine- induced abnormal behaviour. Psychopharmacologia, 7 (1965) 416–422.CrossRefGoogle Scholar
  29. 29.
    Simon, J.R., Martin, D.L., and Kroll, M.J., Sodium-dependent efflux and exchange of GABA in synaptosomes, J. Neurochem., 23 (1974) 981–991.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • J. S. de Belleroche
    • 1
  • H. F. Bradford
    • 1
  1. 1.Department of BiochemistryImperial College of Science and TechnologyLondonGreat Britain

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