Genetic Manipulations with Plant Material pp 579-580 | Cite as
Ribonucleic Acid Synthesis in Plants
Abstract
The two large RNA components of the ribosomes of the plant cytoplasm have molecular weights of 1.3 and 0.7 x 106. These molecules are synthesized in the nucleolus as part of a polycistronic precursor which is subsequently methylated and cleaved in a number of processing steps to produce functional rRNA (1). Polyacrylamide gel electrophoresis of pulse-labelled RNA from the nuclei of aseptically grown Phaseolus aureus seedlings shows that the rRNA precursors and processing intermediates have molecular weights of approximately 2.6, 1.45, and 1.0 x 106. The precursors have a similar base composition to rRNA and competitive hybridi-zation experiments show that the large precursor contains sequences for both the 1.3 and 0.7 x 106 molecular weight rRNAs (2). Saturation hybridization experiments between rRNA and nuclear DNA shows that 0.6–0.9% of the DNA codes for rRNA.
Keywords
Hybridization Experiment Processing Intermediate Acer Pseudoplatanus Large Precursor Diploid NucleusReference
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