Studies on Protease Sensitive Transfecting DNA of Bacillus Phage

  • Hideo Hirokawa
Chapter
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 3)

Abstract

The results of physical analysis of DNA indicated that transfective DNA of Bacillus phage Ø29 bound a protein (1). This DNA-protein complex was further analyzed with labelled DNA (3H)-protein (35S of 14C). The profile of density distribution of this complex gave a single peak in CsC1 density centrifugation. When the DNA was separated into single stranded molecules, a protein was still bound to both strands. The peak of the DNA in CsC1 centrifugation shifted to heavy positions either by trypsin or by S1 (single-endonuclease) treatment. Thus treated DNA lost their transfecting actibity. Sedimentation patterns of the DNA in sucrose density centrifugation did not alter at the main peak before and after trypsin treatment (1).

Keywords

Density Distribution Main Peak Single Peak Genetic Manipulation Sedimentation Pattern 
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Reference

  1. (1).
    Hirokawa, H. 1972. Proc. Nat. Acad. Sci. U.S.A. 69: 1555.CrossRefGoogle Scholar
  2. (2).
    Arwert, F. and G. Venema. 1974. J. Virology 13: 584–589.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Hideo Hirokawa
    • 1
  1. 1.Laboratory of Genetics, Faculty of ScienceUniversity of TokyoHongo, Bunkyo-ku, TokyoJapan

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