In Vitro Strategies for Sandalwood Propagation

  • V. A. Bapat
  • P. S. Rao
Chapter

Abstract

This paper comprises the details of investigations carried out on the regenerative potentialities of organ, callus, cell suspension and protoplast cultures of a tree species Santalum album (Sandalwood). Direct differentiation of multiple buds was observed from hypocotyl and nodal stem segments, whereas callus development and formation of somatic embryos was observed from stem internodal segments of mature 20-year-old trees. Callus tissues also yielded cell suspensions which turned out to be embryo-genie. Endosperm isolated from mature fruits also callused and under appropriate conditions, differentiated somatic embryos. Somatic embryos obtained through such diverse morphogenetic pathways developed into plantlets which were successfully established in the soil. Protoplasts were also successfully isolated from various explant sources. Stem callus protoplasts as well as protoplasts derived from cell suspensions underwent divisions, formed colonies and regenerated somatic embryos. Synthetic seeds were prepared by encapsulating somatic embryos in the alginate matrix. The potentiality of in vitro technology is discussed with respect to sandalwood multiplication and the preservation of germplasm.

Keywords

Somatic Embryo Somatic Embryogenesis Callus Tissue Protoplast Culture Synthetic Seed 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • V. A. Bapat
    • 1
  • P. S. Rao
    • 1
  1. 1.Plant Biotechnology Section. Bio-Organic DivisionBhabha Atomic Research CentreTrombay, BombayIndia

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