Murine Coronavirus 5′-End Genomic RNA Sequence Reveals Mechanism of Leader-Primed Transcription
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Mouse hepatitis virus (MHV) contains a single-strand, positive-sense RNA genome which is transcribed in infected cells, first, into a full-length negative-strand RNA (Brayton et al., 1982; Lai et al., 1982) and then into a positive-sense genomic RNA and six species of subgenomic mRNA. The mRNAs consist of a 3′ co-terminal nested-set structure (Lai et al., 1981), and also contain an identical leader sequence of approximately 72 nucleotides at the 5′ ends (Lai et al., 1984; Spaan et al., 1983). Ultraviolet transcriptional mapping studies (Jacobs et al., 1981) and the fact that no nuclear function is required for replication (Brayton et al., 1981; Wilhelmsen et al., 1981) suggest that the joining of the leader sequences to coronavirus mRNAs does not utilize conventional eukaryotic splicing mechanisms.
KeywordscDNA Clone Leader Sequence Infectious Bronchitis Virus Mouse Hepatitis Virus Avian Infectious Bronchitis Virus
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