Coronaviruses pp 475-481 | Cite as

Antibody Response in Swine to Individual Transmissible Gastroenteritis Virus (TGEV) Proteins

  • Ronald Wesley
  • Roger Woods
  • Paul Kapke
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


Transmissible gastroenteritis virus (TGEV) is a coronavirus that produces a life-threatening diarrhea in neonatal pigs. Whereas sows infected with virulent virus produce protective colostral antibody, sows vaccinated with attenuated virus are significantly less effective in passively protecting baby pigs. Therefore, an understanding of the antigenic and biochemical properties of pathogenic TGEV and the pigs’ immune response to these antigens is a critical area of TGEV research. We are in the process of characterizing the porcine immune response to major TGEV antigens in a protein A immunoprecipitation assay. Protein A is known to bind approximately 90% of the swine immunoglobulin isotypes, IgG1. and IgG2, but is less efficient in binding either swine IgM or IgA.1 This study has led to the serological identification of a new TGEV-specific low molecular weight polypeptide.


Nucleocapsid Protein Convalescent Seron Transmissible Gastroenteritis Virus Feline Infectious Peritonitis Virus Infected Cell Lysate 
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  1. 1.
    R. Lindmark, K. Thoren-Tolling, and J. Sjoquist, Binding of Immunoglobulins to Protein A and Immunoglobulin Levels in Mammalian Sera. J. Immunological Methods, 62: 1–13 (1983).CrossRefGoogle Scholar
  2. 2.
    R. D. Wesley and R. D. Woods, Identification of a 17,000 Molecular Weight Antigenic Polypeptide in Transmissible Gastroenteritis Virus-Infected Cells. J. Gen. Virol. 67: 1419–1425 (1986).PubMedCrossRefGoogle Scholar
  3. 3.
    M. A. Skinner and S. G. Siddell, Coding Sequence of Coronavirus MHV-JHM mRNA 4. J. Gen. Virol. 66: 593–596 (1985).PubMedCrossRefGoogle Scholar
  4. 4.
    M. C. Horzinek, H. Lutz, and N. C. Pedersen, Antigenic Relationships Among Homologous Structural Polypeptides of Porcine, Feline, and Canine Coronaviruses. Infect. Immun. 37: 1148–1155 (1982).PubMedGoogle Scholar
  5. 5.
    S. A. Fiscus, Y. A. Teramoto, M. M. Mildbrand, C. V. Knisley, S. E. Winston, and N. C. Pedersen, Competitive Enzyme Immunoassays for the Rapid Detection of Antibodies to Feline Infectious Peritonitis Virus Polypeptides. J. Clin. Microbiol. 22: 395–401 (1985).PubMedGoogle Scholar
  6. 6.
    M. C. Horzinek, J. Ederveen, H. Egberink, H. E. L. Jacobse-Geels, T. Niewold, and J. Prins, Virion Polypeptide Specificity of Immune Complexes and Antibodies in Cats Inoculated with Feline Infectious Peritonitis Virus. Am. J. Vet. Res. 47: 754–761 (1986).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Ronald Wesley
    • 1
  • Roger Woods
    • 1
  • Paul Kapke
    • 1
  1. 1.US Department of AgricultureNational Animal Disease CenterAmesUSA

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