Coronaviruses pp 365-371 | Cite as

Protective Effect of the F(ab′)2 Fragments of Monoclonal Antibodies to Mouse Hepatitis Virus

  • Kazue Nakanaga
  • Kazuya Yamanouchi
  • Kosaku Fujiwara
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


The F(ab′)2 fragments were prepared from three monoclonal antibodies (MAbs) reactive with either peplomer glycoprotein (E2) or nucleocapsid protein (NP) of a low-virulence mouse hepatitis virus (MHV), MHV-NuU. All the three MAbs could protect mice from challenge infection with virulent MHV-2, whereas only one of the anti-E2 MAbs was capable of neutralizing the virus in vitro. The F(ab′)2 fragment of neutralizing anti-E2 MAb was shown to protect mice from challenge infection, but those of non-neutralizing anti-E2 and anti-NP MAbs were not protective.


Nucleocapsid Protein Challenge Infection Mouse Hepatitis Virus Viral Nucleocapsid Protein Virus Neutralize Titer 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Kazue Nakanaga
    • 1
  • Kazuya Yamanouchi
    • 1
  • Kosaku Fujiwara
    • 1
  1. 1.Department of Animal Pathology, Institute of Medical ScienceUniversity of TokyoMinato-ku, Tokyo 108Japan

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