Protective Effect of the F(ab′)2 Fragments of Monoclonal Antibodies to Mouse Hepatitis Virus
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The F(ab′)2 fragments were prepared from three monoclonal antibodies (MAbs) reactive with either peplomer glycoprotein (E2) or nucleocapsid protein (NP) of a low-virulence mouse hepatitis virus (MHV), MHV-NuU. All the three MAbs could protect mice from challenge infection with virulent MHV-2, whereas only one of the anti-E2 MAbs was capable of neutralizing the virus in vitro. The F(ab′)2 fragment of neutralizing anti-E2 MAb was shown to protect mice from challenge infection, but those of non-neutralizing anti-E2 and anti-NP MAbs were not protective.
KeywordsNucleocapsid Protein Challenge Infection Mouse Hepatitis Virus Viral Nucleocapsid Protein Virus Neutralize Titer
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