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Coronaviruses pp 365-371 | Cite as

Protective Effect of the F(ab′)2 Fragments of Monoclonal Antibodies to Mouse Hepatitis Virus

  • Kazue Nakanaga
  • Kazuya Yamanouchi
  • Kosaku Fujiwara
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)

Abstract

The F(ab′)2 fragments were prepared from three monoclonal antibodies (MAbs) reactive with either peplomer glycoprotein (E2) or nucleocapsid protein (NP) of a low-virulence mouse hepatitis virus (MHV), MHV-NuU. All the three MAbs could protect mice from challenge infection with virulent MHV-2, whereas only one of the anti-E2 MAbs was capable of neutralizing the virus in vitro. The F(ab′)2 fragment of neutralizing anti-E2 MAb was shown to protect mice from challenge infection, but those of non-neutralizing anti-E2 and anti-NP MAbs were not protective.

Keywords

Nucleocapsid Protein Challenge Infection Mouse Hepatitis Virus Viral Nucleocapsid Protein Virus Neutralize Titer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Boere, W.A.M., Benaissa-Trouw, B.J., Harmsen, T., Erich, T., Kraaijeveld, C.A., and Snippe, H., 1985, Mechanisms of monoclonal antibody-mediated protection against virulent Semiliki forest virus, J. Virol., 54: 546.PubMedGoogle Scholar
  2. Ey, P.L., Prowse, S.J., and Jenkin, C.R., 1978, Isolation of pure IgGl, IgG2a, and IgG2b immunoglobulins from mouse serum using protein A-sepharose, Immunochemistry, 15: 429.PubMedCrossRefGoogle Scholar
  3. Fujiwara, K., Takenaka, S., and Shumiya, S., 1976, Carrier state of antibody and viruses in a mouse breeding colony persistently infected with Sendai and mouse hepatitis viruses, Lab. Anim. Sci., 26: 153.PubMedGoogle Scholar
  4. Garvey, J.S., Cremer, N.E., and Sussdorf, D.H., 1977, “Methods in immunology, Third Edition,” W.A. Benjamin, Inc., Advanced Book Program Reading, Massachusetts.Google Scholar
  5. Halstead, S.B., and O′Rourke, E.J., 1977, Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody, J. Exp. Med., 146: 201.PubMedCrossRefGoogle Scholar
  6. Hirano, N., Murakami, T., Taguchi, F., Fujiwara, K., and Matsumoto, M., 1981, Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes, Arch. Virol., 70: 69.PubMedCrossRefGoogle Scholar
  7. Hirano, N., Tamura, T., Taguchi, F., Ueda, K., and Fujiwara, K., 1975, Isolation of low-virulent mouse hepatitis virus from nude mice with wasting syndrome and hepatitis, Jpn. J. Exp. Med., 45: 429.PubMedGoogle Scholar
  8. Laemmli, U.K., 1970, Cleavage of the structural proteins during the assembly of the head of bacteriophage T4, Nature (London), 227: 680.CrossRefGoogle Scholar
  9. Lefrancois, L., 1984, Protection against lethal viral infection by neutralizing and nonneutralizing monoclonal antibodies: Distinct mechanisms of action in vivo, J. Virol., 51: 208.PubMedGoogle Scholar
  10. Mathews, J.H., Roehrig, J.T., and Trent, D.W., 1985, Role of complement and the Fc portion of immunoglobulin G in immunity to Venezuelan equine encephalomyelitis virus infection with glycoprotein-specific monoclonal antibodies, J. Virol., 55: 594.PubMedGoogle Scholar
  11. Nakanaga, K., Ishida, T., and Fujiwara, K., 1983, Difference in antibody production against mouse hepatitis virus (MHV) among mouse strains, Lab. Anim., 17: 90.PubMedCrossRefGoogle Scholar
  12. Nakanaga, K., Kitagawa, T., Kiuchi, Y., Makino, S., Hayami, M., and Fujiwara, K., 1985, Production and characterization of monoclonal antibodies to mouse hepatitis virus, MHV-NuU, Jpn. J. Vet. Sci., 47: 423.CrossRefGoogle Scholar
  13. Nakanaga, K., Yamanouchi, K., and Fujiwara, K., 1986, Protective effect of monoclonal antibodies on lethal mouse hepatitis virus infection in mice, J. Virol., 59: 168.PubMedGoogle Scholar
  14. Nelson, J.B., 1952, Acute hepatitis associated with mouse leukemia. I. Pathological features and transmission of the disease, J. Exp. Med., 96: 293.PubMedCrossRefGoogle Scholar
  15. Peiris, J.S.M., Porterfield, J.S., and Roehrig, J.T., 1982, Monoclonal antibodies against the flavivirus West Nile, J. Gen. Virol., 58: 283.PubMedCrossRefGoogle Scholar
  16. Piazza, M., 1969, “Experimental viral hepatitis,” Charles, C. Thomas, Publisher, Springfield, I11.Google Scholar
  17. Robb, J.A., and Bond, C.W., 1979, Coronaviridae, Compr. Virol., 14: 193.CrossRefGoogle Scholar
  18. Rawls, W.E., and Tompkins, W.A.F., 1975, Destruction of virus-infected cells by antibody and complement, in: “Viral immunology and immuno-pathology,” A.L. Notkins, ed., Academic Press, Inc., New York.Google Scholar
  19. Siddell, S., Anderson, R., Cavanagh, D., Fujiwara, K., Klenk, H.D., Macnaughton, M.R., Pensaert, M., Stohlman, S.A., Sturman, L.S., and van der Zeijst, B.A.M., 1983, Coronaviridae, Intervirology, 20: 181.CrossRefGoogle Scholar
  20. Siddell, S., Wege, H., and ter Meulen, V., 1982, The structure and replication of coronaviruses, Curr. Top. Microbiol. Immunol., 99: 131.PubMedCrossRefGoogle Scholar
  21. Sturman, L.S., and Holmes, K.V., 1983, The molecular biology of corona-viruses, Adv. Virus Res., 28: 35.PubMedCrossRefGoogle Scholar
  22. Taguchi, F., Goto, Y., Aiuchi, M., Hayashi, T., and Fujiwara, K., 1979, Pathogenesis of mouse hepatitis virus infection. The role of nasal epithelial cells as a primary target of low-virulence virus, MHV-S, Microbiol. Immunol., 23: 246.Google Scholar
  23. Taguchi, F., Makino, S., and Fujiwara, K., 1982, Antigenic differentiation of mouse hepatitis viruses by neutralization test, Microbiol. Immunol., 26: 741.PubMedGoogle Scholar
  24. Townsend, A.R.M., and Skehel, J.J., 1984, The influenza A virus nucleo-protein gene controls the induction of both subtype specific and cross-reactive cytotoxic T cells, J, Exp. Med., 160: 552.CrossRefGoogle Scholar
  25. Wege, H., Siddell, S., and ter Meulen, V., 1982, The biology and pathogenesis of coronaviruses, Curr. Top. Microbiol. Immunol., 99: 165.PubMedCrossRefGoogle Scholar
  26. Yewdell, J.W., Bennink, J.R., Smith, G.L., and Moss, B., 1985, Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes, Proc. Natl. Acad. Sci. USA, 82: 1785.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Kazue Nakanaga
    • 1
  • Kazuya Yamanouchi
    • 1
  • Kosaku Fujiwara
    • 1
  1. 1.Department of Animal Pathology, Institute of Medical ScienceUniversity of TokyoMinato-ku, Tokyo 108Japan

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