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Coronaviruses pp 255-263 | Cite as

Characterization of a Non-Pathogenic MHV3 Variant Derived from a Persistently Infected Lymphoid Cell Line

  • L. Lamontagne
  • J. M. Dupuy
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)

Abstract

Mouse hepatitis virus type 3 infection leads to various types of evolution according to strain, age and immune status of animals-1, 2. Three types of viral sensitivity were observed: resistance, full susceptibility and semisusceptibility. The latter is characterized by a chronic disease with occurrence of paralysis, immunodepression, and viral persistency since MHV3 can be recovered during the first three months postinfection in most animals from brain, liver, spleen and lymph nodes1. During the first four days of infection, virus was recovered from the liver of resistant as well as susceptible strain mice. In resistant A/J strain mice, viral titers were consistently low whereas titers greater than 104 were always found in susceptible C57BL/6 animals. In the resistant mouse strain, virus was cleared from liver, brain and serum within seven days. In contrast, virus continued to replicate until death in susceptible animals. Immunosuppressive treatment, however, can abogate the resistance displayed by adult A/J mice, leading to an acute disease and to death. Protection of susceptible newborn mice against MHV3 infection requires the transfer of several cell populations originating from syngenic adult donors: adherent spleen cells, T lymphocytes and a third population present in the non-adherent spleen cell fraction as well as in peritoneal exudate and in bone marrow cells2, 3. Resistance to the acute disease was also correlated with viral replication in hepatocytes4, in embryonic fibroblasts5 and in peritoneal exudate cells2, 6, 7. Recent work suggested that at least two complementary mechanisms were required to confer resistance to MHV3: resistance genes operating at the macrophage level and cells capable to elicit an efficient immune response8.

Keywords

Virus Titer Peritoneal Exudate Susceptible Mouse Mouse Hepatitis Virus Lymphoid Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    C. Le Prévost, E. Levy-Leblond, J.L. Virelizier, and J.M. Dupuy, Immunopathology of mouse hepatitis virus type 3 infection. I. Clinical and virological observation of a persistent 3 infection, J. Immunol., 115: 640 (1975).PubMedGoogle Scholar
  2. 2.
    J.M. Dupuy, E. Levy-Leblond, and C. Le Prévost, Immunopathology of mouse hepatitis virus type 3 infection. II. Effect of immunosuppression in resistant mice, J. Immunol., 114-226 (1975).Google Scholar
  3. 3.
    M. Tardieu, C. Hery, and J.M. Dupuy, Neonatal susceptibility to MHV3 infection in mice. II. Role of natural effector marrow cells in transfer of resistance, J. Immunol., 124: 418 (1980).PubMedGoogle Scholar
  4. 4.
    H. Arnheiter, T. Baechi, and O. Haller, Adult mouse hepatocytes in primary monolayer culture express genetic resistance to mouse hepatitis virus type 3, J. Immunol., 129: 1275 (1982).PubMedGoogle Scholar
  5. 5.
    L.M. Lamontagne and J.M. Dupuy, Natural resistance of mice to mouse hepatitis virus type 3 infection is expressed in embryonic fibroblast cells, J. Gen. Virol., 65: 1165 (1984).PubMedCrossRefGoogle Scholar
  6. 6.
    J.L. Virelizier and A.C. Allison, Correlation of persistent mouse hepatitis virus (MHV3) infection with its effect on mouse macrophage cultures, Arch. Virol., 50: 279 (1976).PubMedCrossRefGoogle Scholar
  7. 7.
    M.R. MacNaughton and S. Patterson, Mouse hepatitis virus strain 3 infection of C5, A/S and A/J strain mice and their macrophages, Arch. Virol., 66: 71 (1980).PubMedCrossRefGoogle Scholar
  8. 8.
    C. Dupuy, D. Lafforet-Cresteil, and J.M. Dupuy, Genetic study of MHV3 infection in mice: in vitro replication of virus in macrophages, in: “Genetic control of natural resistance to infection and malignancy”, E. Skamene, P.A.L. Kongshavn and M. Landry, eds, Academic Press, New York (1980).Google Scholar
  9. 9.
    L.M. Lamontagne and J.M. Dupuy, Persistent infection with mouse hepatitis virus 3 in mouse lymphoid cell lines, Infect. & Immun., 44: 716 (1984).Google Scholar
  10. 10.
    H. Towbin, T. Staehlin, and J. Gordon, Electrophoretic transfer or proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci USA, 76: 4350 (1979).PubMedCrossRefGoogle Scholar
  11. 11.
    J.O. Fleming, S.A. Stohlman, R.C. Harmon, M.M.C. Lai, J.A. Frelinger and L.P. Weiner, Antigenic relationships of murine coronaviruses: analysis using monoclonal antibodies to JHM (MHV4) virus, Virology, 131: 296 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    M.M.C. Lai and S.A. Stohlman, Genome structure of mouse hepatitis virus: comparative analysis by oligo nucleotide mapping, in: “Biochemistry and biology of coronaviruses”, V. Ter Meulen, S. Siddell and H. Wege, eds, Plenum Press, New York (1981).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • L. Lamontagne
    • 1
    • 2
  • J. M. Dupuy
    • 1
    • 2
  1. 1.Dépt. Sc. BiologiquesUniversité du Québec à MontréalMontréalCanada
  2. 2.Immunology research centreInstitut Armand FrappierLavalCanada

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