Coronaviruses pp 239-254 | Cite as

In Vivo and In Vitro Models of Demyelinating Disease XXI: Relationship Between Differentiation of RAT Oligodendrocytes and Control of JHMV Replication

  • Sven Beushausen
  • Samuel Dales
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


Coronavirus (CV) infection in the central nervous system (CNS) of rodents, first described by Bailey et al. (1), is under investigation by many laboratories as a model of virus-induced demyelinating disease (10, 25, 38). One of the most intriguing factors influencing the disease process in rats is the development of an age-related resistance to the neurotropic CV strain JHM virus (JHMV) which becomes manifested at about the time of weaning, when the animal is 3 weeks old and myelination of the CNS is being completed (37). When tested in vitro in primary expiants of neural cells from the neonatal rat CNS, replication of JHMV can be initiated in recently established oligodendrocyte cultures but is repressed in 3 week old cultures, coincident with their “time-clock” of differentiation. Experimentally manipulated differentiation of cultured oligodendrocytes by treatment with cAMP analogues such as N6, 2′ -0-dibutyryl-adenosine 3′:5′ -cyclic monphosphate (dbcAMP) or metabolites affecting the levels of cAMP also results in suppression of JHMV replication (3). This implies that metabolic events leading to the development towards the terminally differentiated cell may be involved in regulating virus expression. Control over virus expression by regulation of cAMP levels is not unique to either the CV or the oligodendrocyte: it has been shown to occur in other virus-cell systems, including infections of neuronal and non-neuronal cells by measles virus (24, 31, 46), and rat glial cells by rubella virus (41).


Measle Virus Demyelinating Disease Nucleocapsid Protein Rubella Virus Cyclic Monophosphate 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Sven Beushausen
    • 1
  • Samuel Dales
    • 1
  1. 1.Cytobiology Group, Department of Microbiology and ImmunologyUniversity of Western OntarioLondonCanada

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