Coronaviruses pp 155-156 | Cite as

Translation and Processing of MHV-A59 Virion RNA in Reticulocyte Lysates and Infected Cells

  • Mark Denison
  • Stanley Perlman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 218)


The first event in the replication of coronavirus MHV-A59, after adsorption, penetration and uncoating, must be the translation of virion RNA into an RNA-dependent RNA polymerase. The study of RNA polymerase activity in infected cells, and the identification of the proteins responsible for that activity is difficult because the protein is synthesized in very small quantities. To circumvent that problem, we have used a cell-free translation system from rabbit reticulocytes to identify the proteins coded for by input genomic RNA. We have also confirmed the validity of this approach by identifying the major in vitro translation product in infected L-2 cells.


Infected Cell High Performance Liquid Chromatography System Rabbit Reticulocyte Lysate Molecular Weight Product Performic Acid 
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  1. Franssen, H, Goldbach, R, van Kämmen, A. 1984. J. Virol. 50: 183PubMedGoogle Scholar
  2. Leibowitz, J, Weiss, S, Paavola, E, Bond, C. 1982. J. Virol. 43: 905PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Mark Denison
    • 1
  • Stanley Perlman
    • 1
  1. 1.Department of PediatricsUniversity of IowaIowa CityUSA

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