Translation and Processing of MHV-A59 Virion RNA in Reticulocyte Lysates and Infected Cells
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The first event in the replication of coronavirus MHV-A59, after adsorption, penetration and uncoating, must be the translation of virion RNA into an RNA-dependent RNA polymerase. The study of RNA polymerase activity in infected cells, and the identification of the proteins responsible for that activity is difficult because the protein is synthesized in very small quantities. To circumvent that problem, we have used a cell-free translation system from rabbit reticulocytes to identify the proteins coded for by input genomic RNA. We have also confirmed the validity of this approach by identifying the major in vitro translation product in infected L-2 cells.