Electron-Microscopic Studies on Cerebrospinal Fluid Sediment

  • Robert M. Herndon
Chapter

Abstract

In a brilliant series of reports beginning with his introduction of lumbar puncture in 1891, Quincke12 described the normal cerebrospinal fluid (CSF) and most of the tests that we routinely perform today. He described the increased cell count in serous meningitis and the increased cell count, low sugar, and bacteria in bacterial meningitis. In addition, he described the changes in sugar, protein, and cell count and the presence of tubercle bacilli in the pellicle in tuberculous meningitis. Early cytological studies were carried out by Quincke and a number of other investigators, but the field was largely neglected until exfoliative cytology, in general, expanded with the introduction of the Papanicolaou stain in 1942.11 Since then, the cytological examination of CSF has been carried out in an increasing number of centers. Results have, in general, been rather disappointing and have, with a few exceptions, been limited to the study of malignancies. More recently, these studies have included special stains for ribonucleic acid, studies of thymidine uptake by CSF cells, and studies of cultured cells.9,14,17 In addition, immunofluorescent stains have been used to detect infectious agents in CSF.16

Keywords

Myelin Basic Protein Bacterial Meningitis Demyelinating Disease Tuberculous Meningitis Mumps Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Cohen, S., Brune, M. J., Herndon, R. M., McKhann, J. M: Cerebrospinal fluid myelin basic protein and multiple sclerosis. In J. Palo (ed.): Myelination and Demyelination. Adv. Exp. Med. Biol.100:513–520, 1978.Google Scholar
  2. 2.
    Cohen, S. R., Brune, M. J., Herndon, R. M., McKhann, G. M: Diagnostic value of myelin basic protein in cerebrospinal fluid. Ann. Neurol. (in press).Google Scholar
  3. 3.
    Duffy, P. E., Simon, J., Defendini, R., Karalian, S.: The study of cells in cerebrospinal fluid by electron microscopy. Arch. Neurol.21:358–362, 1969.CrossRefGoogle Scholar
  4. 4.
    Herndon, R. M., Johnson, M.: A method for the electron microscopic study of cerebrospinal fluid sediment. J. Neuropathol. Exp. Neurol.29:320–330, 1970.CrossRefGoogle Scholar
  5. 5.
    Herndon, R. M., Johnson, R. T., Davis, L. E., Descalzi, L. R. Ependymitis in mumps virus meningitis: Electron microscopical studies of cerebrospinal fluid. Arch. Neurol.30:475–479, 1974.CrossRefGoogle Scholar
  6. 6.
    Herndon, R. M., Kasckow, J.: Electron microscopic studies of cerebrospinal fluid sediment in demyelinating disease. Ann. Neurol.4:515–523, 1978.CrossRefGoogle Scholar
  7. 7.
    Ito, U., Inaba, Y.: Electron microscopic observation of cerebrospinal fluid cells—A new method for embedding of CSF cells. J. Electron Microsc.19:265–270, 1970.Google Scholar
  8. 8.
    Johnston, W. W., Ginn, F. L., Amatulli, J. M.: Light and electron microscopic observations on malignant cells in cerebrospinal fluid from metastatic alveolar cell carcinoma. Acta Cytol.15:365–371, 1971.Google Scholar
  9. 9.
    Kolmel, H. W., Chone, B. K. F.: Combined application of cytodiagnostic technics to the cerebrospinal fluid. J. Neurol.216:1–8, 1977.CrossRefGoogle Scholar
  10. 10.
    Luft, J. H.: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol.9:409–414, 1961.CrossRefGoogle Scholar
  11. 11.
    Papanicolaou, G. N.: A new procedure for staining vaginal smears. Science95:438–439, 1942.CrossRefGoogle Scholar
  12. 12.
    Quincke, H. I.: Ueber Hydrocephalus. Verh. Kong. Innere Med. Wiesb., pp. 321-331, 1891.Google Scholar
  13. 13.
    Rambourg, A.: An improved silver methenamine technique for the detection of periodic acid reactive complex carbohydrates with the electron microscope. J. Histochem. Cytochem.15:409–412, 1967.CrossRefGoogle Scholar
  14. 14.
    Schmidt, R. M., Schmidt, T., Seifert, B.: Beitrag zur Fluorescenz und Elektronen Mikroskopie der Liquorzellen. Psychiatr. Neurol. Med. Psychol.18:201–203, 1966.Google Scholar
  15. 15.
    Schmidt, R. M., Seifert, B.: Beitrag zur ultrastrukturellen Darstellung der Liquorzelle. Dtsch. Z. Nervenheilkd.192:209–225, 1967.CrossRefGoogle Scholar
  16. 16.
    Sommerville, R. G.: Rapid identification of neurotropic viruses by an immunofluorescent technique applied to cerebrospinal fluid cellular deposits. Arch. Gesamte Virusforsch.19:63–69, 1966.CrossRefGoogle Scholar
  17. 17.
    Tourtellotte, W. W.: A selected review of reactions of the cerebrospinal fluid to disease. In Fields, W. S. (ed.): Neurological Diagnostic Techniques. Springfield, Illinois, Charles C. Thomas, 1966, pp. 25–49.Google Scholar
  18. 18.
    Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25:407–408, 1965.CrossRefGoogle Scholar
  19. 19.
    Watson, M.: Staining of tissue sections for electron microscopy with heavy metals. J. Biophys. Biochem. Cytol.4:475–478, 1958.CrossRefGoogle Scholar
  20. 20.
    Wender, M., Sniatala: Zur Feinstructur der Liquorzellen. Wien. Z. Nervenheilkd.27:38–44, 1969.Google Scholar
  21. 21.
    Westrum, L. E.: A combination staining technique for electron microscopy. J. Microsc.4:275–278, 1965.Google Scholar
  22. 22.
    Whitaker, J. N.: Myelin encephalitogenic protein fragments in cerebrospinal fluid of persons with multiple sclerosis. Neurology27:911–920, 1977.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Robert M. Herndon
    • 1
  1. 1.Center for Brain Research and Department of NeurologyUniversity of Rochester School of Medicine and DentistryRochesterUSA

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