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Distinction of Receptor from Nonreceptor Interactions in Binding Studies

  • Morley D. Hollenberg
  • Pedro Cuatrecasas
Part of the The Receptors book series (REC, volume 1)

Abstract

Studies of the binding of ligands to various putative pharmacologic receptors are entering a new phase. Over the past decade, sufficient expertise has been developed to measure with confidence the binding of highly radioactively labeled probes to quantitatively small numbers of high-affinity binding sites, which in most cases represent pharmacologic receptors of interest. Now, these techniques are being employed to study receptors for an ever-burgeoning number of pharmacologically active agents [Cuatrecasas and Hollenberg (1976); collection of reviews edited by Blecher (1976)]. Furthermore, it is becoming apparent that the task of assessing receptor characteristics by ligand binding studies (e.g., receptor changes related to desensitization phenomena) may require a more sophisticated approach than may have been initially considered adequate. It thus appears appropriate to develop an overview for such studies, particularly from the point of view of distinguishing ligand binding sites which typify “true” receptor characteristics (therefore specific binding sites) from those cellular binding sites which do not reflect receptor properties (therefore nonspecific or nonreceptor binding sites).

Keywords

Human Serum Albumin Ligand Affinity Native Insulin Pharmacologic Receptor Label Ligand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • Morley D. Hollenberg
    • 1
  • Pedro Cuatrecasas
    • 2
  1. 1.Division of Clinical Pharmacology, Departments of Medicine and of Pharmacology and Experimental TherapeuticsThe Johns Hopkins University School of MedicineBaltimoreUSA
  2. 2.The Wellcome Research LaboratoriesBurroughs Wellcome CompanyResearch Triangle ParkUSA

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