Neoplastic Transformation: The Relevance of In Vitro Studies for the Understanding of Tumor Pathenogenesis and Neoplastic Growth

  • L. A. Smets
Chapter
Part of the NATO Advanced Study Institutes Series book series (NSSA, volume 21b)

Abstract

Cell culture studies are indispensable for the understanding of normal and aberrant growth behaviour. They offer a vast spectrum of experimental and analytical possibilities at the cellular level which are difficultly provided for by in vivo experimentation.

Keywords

Cell Transformation NEOPLASTIC Transformation Membrane Glycoprotein Morphological Transformation Semisolid Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Eagle, H. (1965). “Metabolic controls in cultured mammalian cells.” Science 148, 42.PubMedCrossRefGoogle Scholar
  2. 2.
    Hoelzer, D., Kurrle, E., Ertl, U. and Milewsky, A. (1974). “Growth of human leu kaemic peripheral blood cells in diffusion chambers.” Europ. J. Cancer 10, 579.CrossRefGoogle Scholar
  3. 3.
    Furmansky, P., Silverman, D.J. and Lubin, M. (1971). “Expression of differentiated fuctions in mouse neuroblastoma mediated by dibutyryl-cyclic adenosine monophosphate.” Nature 233, 415.CrossRefGoogle Scholar
  4. 4.
    Currie, G. and Bagshawe, K. (1967). “The masking of antigens on throphoblasts and cancer cells.” Lancet 1, 708.PubMedCrossRefGoogle Scholar
  5. 5.
    Smets, L.A. and Broekhuysen-Davies, J. (1972). “Shielding of antigens and Concanavalin A agglutination sites by surface coat of transplantable mouse lymphosarcoma cells.“ Europ. J. Cancer 8, 541.CrossRefGoogle Scholar
  6. 6.
    Smets, L.A. and Homburg, Ch. (1976). “Immunogenicity and Concanavalin A agglutinability in transplantable mouse lymphosarcoma and human leukemia.” Europ. J. Cancer 12, 605.CrossRefGoogle Scholar
  7. 7.
    Jones, P.D.E. and Castro, J.E. (1977). “Immunological mechanisms in metastatic spread and the antimetastatic effect of C. parvum.” Br. J. Cancer 35, 519.PubMedCrossRefGoogle Scholar
  8. 8.
    Leavitt, J.C., Crawford, B.D., Barrett, J.C. and Ts’o, P.O. (1977). “Regulation of requirements for anchorage independent growth of Syrian hamster fibroblasts by somatic mutation.” Nature 268, 63.CrossRefGoogle Scholar
  9. 9.
    Taylor, J.C., Hill, D.W. and Rogolsky, M. (1975). “Purine and pyrimidine modification of growth and surface properties of baby hamster kidney cells (BHK21/C13).” Exptl. Cell Res. 90, 468.PubMedCrossRefGoogle Scholar
  10. 10.
    Emmelot, P. (1973). ’’Biochemical properties of normal and neoplastic surfaces; a review.“ Europ. J. Cancer 9, 319.CrossRefGoogle Scholar
  11. 11.
    Nicolson, G.L. (1976). “Trans-membrane control of the receptors on normal and tumor cells. II. Surface changes associated with transformation and malignancy.” Biochim. Biophys. Acta 458, 1.PubMedGoogle Scholar
  12. 12.
    Smith, D.E. and Walborg Jr., E.F. (1977). “The tumor cell peripery: carbohydrate components.” In: Mammalian cell membranes, G. Jamieson and D. Robinson, Eds., Vol. 3, p. 115. Butterworth, Boston.Google Scholar
  13. 13.
    Burger, M. (1970). “Proteolytic enzymes initiating cell division and escape from contact inhibition of growth.” Nature 227, 170.PubMedCrossRefGoogle Scholar
  14. 14.
    Smets, L.A., De Ley, L. and Collard, J.G. (1975). “Contact-mediated changes in cytoagglutination.” Exptl. Cell Res. 95, 95.PubMedCrossRefGoogle Scholar
  15. 15.
    Willingham, M.C. and Pastan, I. (1975). “Cyclic AMP modulates microvillus formation and agglutinability in transformed and normal mouse fibroblasts.” Proc. Natl. Acad. Sci. USA 72, 1263.PubMedCrossRefGoogle Scholar
  16. 16.
    Temmink, J.H. and Collard, J.G. (1977). “Cell surface microvilli and cell agglutinability.” Cell Biol. Int. Rep. 1, 169.PubMedCrossRefGoogle Scholar
  17. 17.
    Barnett, R.E., Furcht, L.T. and Scott, R.E. (1974). “Differences in membrane fluidity and structure in contact-inhibited and transformed cells.” Proc. Natl. Acad. Sci. USA 71, 1992.Google Scholar
  18. 18.
    Horwitz, A., Hatten, M.E. and Burger, M.M. (1974). “Membrane fatty acid replacements and their effect on growth and lectin induced agglutinability.” Proc. Natl. Acad. Sci. USA 71, 3115.PubMedCrossRefGoogle Scholar
  19. 19.
    Collard, J.G., De Wildt, A., Oomen-Meulemans, E.P.M., Smeekens, J. and Emmelot, P. (1977). “Increase in fluidity of membrane lipids in lymphocytes, fibroblasts and liver cells stimulated for growth.” FEBS Lett. 77, 173.PubMedCrossRefGoogle Scholar
  20. 20.
    Hakamori, S. (1975). “Structures and organization of cell surface glycolipids. Dependency on cell growth and malignant transformation.” Biochim. Biophys. Acta 417, 55.Google Scholar
  21. 21.
    Steiner, S., Brennan, P. and Melnick, J. (1973). “Fucosyl-glycolipid metabolism in oncornavirus-transformed cell lines.” Nature New Biol. 245, 19.PubMedGoogle Scholar
  22. 22.
    Vaheri, A. and Ruoslahti, E. (1974). “Disappearance of major cell-type specific surface glycoprotein antigen (SF) after transformation of fibroblasts by Rous sarcoma virus.” Int. J. Cancer 13, 579.PubMedCrossRefGoogle Scholar
  23. 23.
    Warren, L., Fuhrer, J.P. and Buck, C.A. (1972). “Surface glycoproteins of normal and transformed cells: A difference determined by sialic acid and a growth dependent sialyl transfererase.” Proc. Natl. Acad. Sci. USA 69, 1838.PubMedCrossRefGoogle Scholar
  24. 24.
    Van Beek, W.P., Smets, L.A. and Emmelot, P. (1973). “Increased sialic acid density in surface glycoprotein of transformed and malignant cells - A general phenomenon?” Cancer Res. 33, 2913.PubMedGoogle Scholar
  25. 25.
    Van Beek, W.P., Smets, L.A. and Emmelot, P. (1975). “Changed surface glycoprotein as a marker of malignancy in human leukaemic cells.” Nature 253, 457.PubMedCrossRefGoogle Scholar
  26. 26.
    Van Beek, W.P., Emmelot, P. and Homburg, C. (1977). “Comparison of cell-surface glycoproteins of rat hepatomas and embryonic rat liver.” Br. J. Cancer 36, 157PubMedCrossRefGoogle Scholar
  27. 27.
    Van Beek, W.P., Smets, L.A., Emmelot, P., Roozendaal, K.J. and Behrendt, H. (1978). “Early recognition of human leukemia by cell surface glycoprotein changes.” Leukemia Res. in press.Google Scholar
  28. 28.
    Chritchley, D.R., Wyke, J.A. and Hynes, R. (1975). “Cell surface and metabolic labelling of the proteins of normal and transformed chicken cells.” Biochim. Biophys. Acta 436, 335.Google Scholar
  29. 29.
    Van Nest, G.A. and Grimes, W.J. (1977). “A comparison of membrane components of normal and transformed BALB/c cells.” Biochemistry 16, 2092.Google Scholar
  30. 30.
    Shields, R. (1976). “Transformation and tumorigenicity.” Nature 262, 348.CrossRefGoogle Scholar
  31. 31.
    Franks, L.M. and Wilson, P. (1970). “Spontaneous neoplastic transformation in vitro: the ultrastructure of the tissue culture cell.” Europ. J. Cancer 6, 517.CrossRefGoogle Scholar
  32. 32.
    Boone, C.W., Takeichi, N., Paranjpe, M. and Gilden, R. (1976). “Vasoformative sarcomas arising from Balb/3T3 cells attached to solid substrates.” Cancer Res. 36, 1626.PubMedGoogle Scholar
  33. 33.
    Smets, L.A. (1972). “Contact inhibition of transformed cells imcompletely restored by dibutyryl cyclic NIP.” Nature New Biol. 239, 123.PubMedGoogle Scholar
  34. 34.
    Collard, J.G. and Smets, L.A. (1974). “Effect of proteolytic inhibitors on growth and surface architecture of normal and transformed cells.” Exptl. Cell Res. 86, 75.PubMedCrossRefGoogle Scholar
  35. 35.
    Freedman, V.H. and Shin, S. (1977). “Isolation of human diploid cell variants with enhanced colony forming efficiency in semisolid medium after a single-step chemical mutagenesis: Brief communication.” J. Natl. Cancer Inst. 58, 1873.PubMedGoogle Scholar
  36. 36.
    Shin, S., Freedman, V.H., Risser, R. and Pollack, R. (1975). “Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro.” Proc. NatZ. Acad. Sci. USA 72, 4435.CrossRefGoogle Scholar
  37. 37.
    Jones, P.A., Rhim, J.S., Isaacs Jr., H. and McAllister, R.M. (1975). “The relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells.” Int. J. Cancer 16, 616.PubMedCrossRefGoogle Scholar
  38. 38.
    Ceccarini, C. (1975). “Appearance of smaller mannosyl-glycopeptides on the surface of a human cell transformed by simian virus 40.” Proc. Natl. Acad. Sci. USA 72, 2687.PubMedCrossRefGoogle Scholar
  39. 39.
    Smets, L.A., Van Beek, W.P. and Van Rooy, H. (1976). “Surface glycoproteins and Concanavalin-A-mediated agglutinability of clonal variants and tumour cells derived from SV40-virus-transformed mouse 3T3 cells.” Int. J. Cancer 18, 462.PubMedCrossRefGoogle Scholar
  40. 40.
    Smets, L.A., Van Beek, W.P., Van Rooy, H. and Homburg, Ch. (1978). “The relationship between membrane glycoprotein alterations and anchorage-independent growth in neoplastic transformation.” Cancer Biochem. Biophys. in press.Google Scholar
  41. 41.
    Smets, L.A., Van Beek, W.P. and Van Nie, R. (1977). “Membrane glycoprotein changes in primary mammary tumors associated with autonomous growth.” Cancer Letters 3, 133.PubMedCrossRefGoogle Scholar
  42. 42.
    Yaniv, A., Gotlieb-Stematsky, T. and Eylan, E. (1977). “Tumor-specific transplantation antigens in spontaneously transformed hamster cell lines.” Cancer 39, 1450.PubMedCrossRefGoogle Scholar
  43. 43.
    Stiles, C.D., Desmond Jr., W., Sato, G. and Saier Jr., F.H. (1975). “Failure of human cells transformed by simian virus 40 to form tumors in athymic nude mice.” Proc. Natl. Acad. Sci. USA 72, 4971.PubMedCrossRefGoogle Scholar
  44. 44.
    Nilsson, K., Giovanella, B., Stehlin, J. and Klein, G. (1977). “Tumorigenicity of human hemopoietic cell lines in athymic nude mice.” Int. J. Cancer 19, 337.PubMedCrossRefGoogle Scholar
  45. 45.
    Emmelot, P. and Scherer, E. (1977). “Multi-hit kinetics of tumor formation, with special reference to experimental liver and human carcinogenesis and some general conclusions.” Cancer Res. 37, 1702.PubMedGoogle Scholar
  46. 46.
    Ishii, Y., Elliot, J.A., Tishra, N.K. and Lieberman, M.W. (1977). “Quantitative studies of transformation by chemical carcinogens and ultraviolet radiation using a subclone of BHK 21 Clone 13 Syrian hamster cells.” Cancer Res. 37, 2023.Google Scholar
  47. 47.
    Foulds, L. (1969). “Neoplastic development; Vol. I.” Academic press, London.Google Scholar
  48. 48.
    Todaro, G.J. and Green, H. (1966). “Cell growth and the inition of transformation by SV40.” Proc. Natl. Acad. Sci. USA 55, 302.PubMedCrossRefGoogle Scholar
  49. 49.
    Borek, C. and Sachs, L. (1968). “The number of cell generations required to fix the transformed state in X-ray-induced transformation.” Proc. Natl. Acad. Sci. USA 59, 83.PubMedCrossRefGoogle Scholar
  50. 50.
    Nowell, P.C. (1976). “The clonal evolution of tumor cell populations.” Science 194, 23.PubMedCrossRefGoogle Scholar
  51. 51.
    Dahlke, M.B. and Nowell, P.C. (1975). “Chromosomal abnormalities and erythropoiesis in the preleukemic phase of multiple myeloma.” Br. J. Haemato Z. 31, 111.CrossRefGoogle Scholar
  52. 52.
    Berwald, Y. and Sachs, L. (1965). “Transformation of normal cells to tumor cells by carcinogenic hydrocarbons.” J. Natl. Cancer Inst. 35, 641.PubMedGoogle Scholar
  53. 53.
    Pontén, J. (1976). “The relationship between in vitro transformation and tumor formation in vivo.” Biochim. Biophys. Acta 458, 397.PubMedGoogle Scholar
  54. 54.
    Chan, A.C. and Walker, I.G. (1977). “Loss of DNA repair capacity during successive subcultures of primary rat fibroblasts.” J. Cell Biol. 74, 365.PubMedCrossRefGoogle Scholar
  55. 55.
    Todaro, G.J., Nilausen, K. and Green, H. (1963). “Growth properties of polyoma virus induced hamster tumor cells.” Cancer Res. 23, 825.PubMedGoogle Scholar
  56. 56.
    Matsuja, Y., and Yamane, I. (1968). “Serial culture of Syrian hamster fibroblasts in albumin fortified medium and their regular development into established lines.” Exptl. Cell Res. 50, 652.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1979

Authors and Affiliations

  • L. A. Smets
    • 1
  1. 1.The Netherlands Cancer InstituteAmsterdamThe Netherlands

Personalised recommendations