DNA Synthesis pp 613-628 | Cite as
DNA Polymerase in Chromatin
Abstract
Two major types of DNA polymerase from mammalian cells have been described. The DNA polymerase α (6-8S) is the main activity in growing cells. The smaller DNA polymerase β (3-4S) constitutes about 5-15% of the total polymerase activity in growing cells. The roles of each of these polymerase activities in the replication of mammalian chromatin are not yet clear. However, the evidence available indicates that the a polymerase activity is closely correlated with DNA replication in growing L cells (Chang and Bollum, 1973), regenerating rat liver (Baril et al., 1973), in HeLa cells (Spadari and Weissbach, 1974; Chiu and Baril, 1975) and BHK21 cells (Craig et al., 1975). The characteristics of these enzymes have been described in some detail (for review, see Weissbach, 1975; Wintersberg, 1977).
Keywords
Polymerase Activity Sucrose Density Gradient Chromatin Protein Lymphocyte Nucleus Replication EnzymePreview
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