Analysis of Urinary Pregnanediol and Pregnanetriol by Gas-Liquid Chromatography

  • Marvin A. Kirschner
  • Mortimer B. Lipsett
Chapter

Abstract

The measurement of urinary pregnanediol and pregnanetriol are of clinical importance. Methods used previously have generally depended on the formation of sulfuric acid chromogens after purification of the steroid (1–3). The color reaction with sulfuric acid is non-specific and for pregnanediol, sensitivity is poor. We therefore have used GLC for the measurement of pregnanediol and pregnanetriol in urine extracts.

Keywords

Ovarian Cyst Anhydrous Ether Urine Extract Argon Ionization Detector Simple Solvent Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Klopper, A., E. A. Michie, and J. B. Brown, J Endocr 12: 209, 1955.CrossRefGoogle Scholar
  2. 2.
    Bongiovanni, A. M. and W. R. Eberlein, Anal Chem 30: 388, 1958.CrossRefGoogle Scholar
  3. 3.
    Fotherby, K. and D. N. Love, J Endocr 20: 157, 1960.CrossRefGoogle Scholar
  4. 4.
    Haahti, E.O.A., Mercatorin Kirjapaino, Turku, Finland, 1961.Google Scholar
  5. 5.
    Kirschner, M. A. and M. B. Lipsett, Steroids 3: 277, 1964.CrossRefGoogle Scholar
  6. 6.
    VandenHeuvel, W. J. A., B. G. Creech, and E. C. Horning, Anal Biochem 4: 191, 1962.CrossRefGoogle Scholar
  7. 7.
    Turner, D. A., G. E. Seegar-Jones, I. J. Sarlos, A. C. Barnes, and R. Cohen, Anal Biochem 5: 99, 1963.CrossRefGoogle Scholar
  8. 8.
    Patti, A. A., P. Bonanno, T. F. Frawley, and A. A. Stein, Obst & Gynec 21: 302, 1963.Google Scholar
  9. 9.
    Seegar-Jones, G. E., D. Turner, I. J. Sarlos, A. G. Barnes, and R. Cohen, Fertility & Sterility 13: 544, 1962.Google Scholar
  10. 10.
    Patti, A. A., P. Bonanno, T. F. Frawley, and A. A. Stein, Acta Endocr 42: Suppl 77, 1963.Google Scholar
  11. 11.
    Jansen, A. P., Clin Chim Acta 8: 785, 1963.CrossRefGoogle Scholar
  12. l2.
    Cox, R. I. J., J Chromatog 12: 242, 1963.CrossRefGoogle Scholar
  13. 13.
    Cox, R. I. J., J Biol Chem 234: 1693, 1959.Google Scholar
  14. 14.
    Rosenfeld, R. S., M. C. Lebeau, R. D. Jandorek, and T. Salumaa, J Chromatog 8: 355, 1962.CrossRefGoogle Scholar

Copyright information

© Plenum Press 1965

Authors and Affiliations

  • Marvin A. Kirschner
    • 1
  • Mortimer B. Lipsett
    • 1
  1. 1.Endocrinology BranchNational Cancer InstituteBethesdaUSA

Personalised recommendations