Abstract
Autoradiography is a process of photographic image formation, caused by exposure of film to emissions from radioisotopes. Since the image density will be at a maximum close to the source of radiation, autoradiography allows both the detection and localisation of radioactive molecules in a gel, or on the surface of a filter or thin layer chromatography (TLC) plate placed in contact with a sheet of film. Techniques that require autoradiography include protein synthesis in vitro, colony hybridisation, Southern blotting, DNA sequencing, dot blotting, and DNA footprinting (see Chapters 7, 12, 15 and 16 of Volume 1, and Chapters 9 and 15 of this volume).
Keywords
Latent Image Thin Layer Chromatography Plate Silver Atom Sodium Salicylate Image Density
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
Further Reading
- Harnes, B.D. and Rickwood, D. (eds) (1981) Gel Electrophoresis of Proteins: A Practical Approach (IRL Press, London)Google Scholar
- Rickwood, D. and Hames, B.D. (eds) (1982) Gel Electrophoresis of Nucleic Acids: A Practical Approach (IRL Press, London)Google Scholar
References
- 1.Noblette, C.B. (1962) Photography: Its Materials and Processes, 6th edn (Van Nostrand, Princeton, New Jersey)Google Scholar
- 2.Wayne, R.P. (1970) Photochemistry (Butterworths, London)Google Scholar
- 3.Laskey, R.A. (1984) Radioisotope Detection by Fluorography and Intensifying Screens, Review 23 (Amersham International, UK)Google Scholar
- 4.Bonner, W.M. and Laskey, R.A. (1974) A Film Detection Method for Tritium-labelled Proteins and Nucleic Acids in Polyacrylamide Gels, Eur. J. Biochem., 46, 83–88CrossRefGoogle Scholar
- 5.Laskey, R.A. and Mills, A.D. (1977) Enhanced Autoradiographic Detection of 32P and 125I Using Intensifying Screens and Hypersensitized Film, FEBS Lett., 82, 314–316CrossRefGoogle Scholar
- 6.Laskey, R.A. (1980) The Use of Intensifying Screens or Organic Scintillators for Visualizing Radioactive Molecules Resolved by Gel Electrophoresis, in Methods in Enzymology, 65, pp. 363–371 (Academic Press, London)Google Scholar
- 7.Chamberlain, J.P. (1979) Fluorographic Detection of Radioactivity in Polyacrylamide Gels with the Water-soluble Fluor, Sodium Salicylate, Anal. Biochem., 98, 132–135CrossRefGoogle Scholar
- 8.Laskey, R.A. and Mills, A.D. (1975) Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography, Eur. J. Biochem., 56, 335–341CrossRefGoogle Scholar
Copyright information
© John M. Walker and Wim Gaastra 1987