Centrin-Mediated Cell Motility in Algae

  • Michael Melkonian
  • Peter L. Beech
  • Christos Katsaros
  • Dorothee Schulze
Chapter
Part of the Current Phycology book series (CP)

Abstract

Centrin-mediated motility represents a novel cell motility mechanism in eukaryotic cells that has attracted considerable attention in recent years (for reviews see Melkonian, 1989; Salisbury, 1989a,b). The principal protein involved in this type of motility, centrin, was first isolated from an algal flagellate (Salisbury et al., 1984) and since then has been found to be a ubiquitous structural protein of the eukaryotic centrosome (Salisbury et al., 1986; Baron and Salisbury, 1988; Hiraoka et al., 1989). Centrin is a Ca2+-modulated phosphoprotein of the EF-hand protein family and shares significant sequence homologies with other members of this family, including calmodulin and the yeast CDC31 gene product (Salisbury et al., 1984; Huang et al., 1988b; Moncrief et al., 1990). Centrin-mediated cell motility is characterized by contraction of a filamentous structure within less than 20 ms, and much slower reextension of this structure to its original length (in the range of a few seconds up to about 1 h). Contraction of centrin filaments is based on supercoiling, not on sliding, initiated by elevated intracellular Ca2+ levels and is independent of ATP hydrolysis. Reextension of centrin filaments requires removal of Ca2+ from the protein and ATP.

Keywords

Basal Body Basal Apparatus Flagellar Apparatus Cell BioI Forward Swimming 
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References

  1. Amos, W. B. 1971. Reversible mechanochemical cycle in the contraction of Vorticella. Nature 229: 127–128.CrossRefGoogle Scholar
  2. Amos, W. B. 1972. Structure and coiling of the stalk in the peritrich ciliates Vorticella and Carchesium. J. Cell Sci. 10: 95–122.Google Scholar
  3. Amos, W. B. 1975. Contraction and calcium binding in vorticellid ciliates. In R. E. Stephens and S. Inoue (eds.), Molecules and Cell Movement, Raven Press, New York, pp. 411–436.Google Scholar
  4. Amos, W. B., Routledge, L. M., and Yew, F. F. 1975. Calcium-binding proteins in a vorticellid contractile organelle. J. Cell Sci. 19: 203–213.Google Scholar
  5. Andersen, R. A. 1991. The cytoskeleton of chromophyte algae. Protoplasma (in press).Google Scholar
  6. Andersen, R. A., and Wetherbee, R. 1991. Microtubules of the flagellar apparatus are active during prey capture in the chrysophycean alga Epipyxis pulchra. Cell Motil. Cytoskel. (submitted).Google Scholar
  7. Bannister, L. H., and Tatchell, E. C. 1968. Contractility and the fiber systems of Stentor coeruleus. J. Cell Sci. 3: 295–308.Google Scholar
  8. Baron, A. T., Greenwood, T. M., and Salisbury, J. L. 1991. Localization of the centrin-related 165,000-Mr protein of ptK2 cells during the cell cycle. Cell Motil. Cytoskel. 18: 1–14.CrossRefGoogle Scholar
  9. Baron, A. T., and Salisbury, J. L. 1987. Identification and localization of a novel cytoskeletal centrosome associated protein, “centrin,” in PtK2 cells. J. Cell Biol. 105: 205a.Google Scholar
  10. Baron, A. T., and Salisbury, J. L. 1988. Identification and localization of a novel cytoskeletal, centrosome-associated protein in PtK2 cells. J. Cell Biol. 107: 2669–2678.CrossRefGoogle Scholar
  11. Bhattacharya, D., Elwood, H. J., Goff, L. J., and Sogin, M. L. 1990. Phylogeny of Graciliaria lemaneiformis (Rhodophyta) based on sequence analysis of its small subunit ribosomal RNA coding region. J. Phycol. 26: 181–186.CrossRefGoogle Scholar
  12. Cachon, J., and Cachon, M. 1981. Movement by non-actin filament mechanisms. BioSystems 14: 313–326.CrossRefGoogle Scholar
  13. Cachon, J., and Cachon, M. 1984a. An unusual mechanism of cell contraction: Leptodiscinae dinoflagellates. Cell Motil. 4: 41–45.CrossRefGoogle Scholar
  14. Cachon, J., and Cachon M. 1984b. A new Ca2+-dependent function of flagellar rootlets in Dinoflagellates: The releasing of a parasite from its host. Biol. Cell 52: 61–76.Google Scholar
  15. Cachon, J., and Cachon, M. 1985. Non-actin filaments and cell contraction in Kofoidinium and other dinoflagellates. Cell Motil. 5: 1–15.CrossRefGoogle Scholar
  16. Cachon, J., Cachon, M., and Boillot, A. 1983. Flagellar rootlets as myonemal elements for pusule contractility in dinoflagellates. Cell Motil. 3: 61.CrossRefGoogle Scholar
  17. Carasso, N., and Favard, P. 1966. Mis en evidence du calcium dans les myonèmes pédonculaires de ciliés péritriches. J. Microsc. 5: 759–770.Google Scholar
  18. Coling, D. E., and Salisbury, J. L. 1987. Purification and characterization of centrin, a novel calicum-modulated contractile protein. J. Cell Biol. 105: 205a.Google Scholar
  19. Dangeard P. A. 1901. Etude comparative de la zoospore et du spermatozoïde. C.R. Hebd. Seances Acad. Sci. 132: 859–861.Google Scholar
  20. Dassler, C. L., Scott, J., and Salisbury, J. L. 1990. Centrin in “primitive” eucaryotes: Centrin homologues of the Rhodophyta. J. Cell Biol. 111: 182a.Google Scholar
  21. Dodge, J. D. 1987. Dinoflagellate ultrastructure and complex organelles: A. General ultrastructure. In F. J. R. Taylor (ed.), The Biology of Dinoflagellates. Blackwell, Oxford, pp. 93–119.Google Scholar
  22. Doflein, F. 1918. Beiträge zur Kenntnis von Bau und Teilung der Protozoenkerne. Zool Anz. 46: 289–306.Google Scholar
  23. Engelmann, T. W. 1875. Contractilität und Doppelbrechung. Pflügers Arch. Eur. J. Physiol. 11: 432–464.CrossRefGoogle Scholar
  24. Entz, G. 1892. Die elastischen und contractilen Elemente der Vorticellen. Math. Naturw. Ber. Ung. 10: 1–48.Google Scholar
  25. Ettienne, E. M. 1970. Control of contractility in Spirostomum by dissociated calcium ions. J. Gen. Physiol. 56: 168–179.CrossRefGoogle Scholar
  26. Ettienne, E. M., and Seltsky, M. 1974. The antagonistic agents on contraction and reextension in the ciliate Spirostomum ambiguum. J. Cell Sci. 16: 377–383.Google Scholar
  27. Ettl, H., and Moestrup, Ø. 1980. Light and electron microscopical studies on Hafniomonas gen. nov. ( Chlorophyceae, Volvocales), a genus resembling Pyramimonas (Prasinophyceae). PL Syst. Evol. 135: 177–210.Google Scholar
  28. Gillott, M. 1990. Phylum Cryptophyta. In L. Margulis, J. O. Corliss, M. Melkonian, and D. J. Chapman (eds.), Handbook of Protoctista, Jones and Bartlett, Boston, pp. 139–151.Google Scholar
  29. Gillott, M., and Gibbs, S. P. 1983. Comparison of the flagellar rootlets and periplast in two marine cryptomonads. Can. J. Bot. 61: 1964–1978.CrossRefGoogle Scholar
  30. Goodenough, U. W. 1983. Motile detergent-extracted cells of Tetrahymena and Chlamydomonas. J. Cell Biol. 96: 1610–1621.CrossRefGoogle Scholar
  31. Greuet, C. 1976. Organisation et fonctionnement du tentacule postérieur d’Erythropsidinium, Pérdinien Warnowiidae, J. Microsc. Biol. Cell. 27: 13a.Google Scholar
  32. Hamburger, C. 1905. Zur Kenntnis der Dunaliella salina und einer Amöbe aus Salinenwasser von Cagliari. Arch. Protistenkd. 6: 111–130.Google Scholar
  33. Hand, W. G., and Schmidt, J. A. 1975. Phototactic orientation by the marine dinoflagellate Gyrodinium dorsum Kofoid. II. Flagellar activity and overall response mechanisms. J. Protozool. 22: 494–498.Google Scholar
  34. Harris, E. H. 1989. The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use. Academic Press, San Diego, 780 pp.Google Scholar
  35. Hiraoka, L., Golden, W., and Magnuson, T. 1989. Spindle pole organization during early mouse development. Dev. Biol. 133: 24–36.CrossRefGoogle Scholar
  36. Hoffman, L. R. 1973. Fertilization in Oedogonium. I. Plasmogamy. J. Phycol. 9: 62–84.Google Scholar
  37. Hoffmann-Berling, H. 1958. Der Mechanismus eines neuen, von der Muskelkontraktion verschiedenen Kontraktionszyklus. Biochim. Biophys. Acta 27: 247–255.CrossRefGoogle Scholar
  38. Hohfeld, I., Otten, J., and Melkonian, M. 1988. Contractile eukaryotic flagella: Centrin is involved. Protoplasma 147: 16–24.CrossRefGoogle Scholar
  39. Hoops, H. J., Wright, R. L., Jarvik, J. W., and Witman, G. B. 1984. Flagellar waveform and rotational orientation in a Chlamydomonas mutant lacking normal striated fibers. J. Cell Biol. 98: 818–824.CrossRefGoogle Scholar
  40. Huang, B., and Mazia, D. 1975. Microtubules and filaments in ciliate contractility. In R. E. Stephens and S. Inouye (eds.), Molecules and Cell Movement, Raven Press, New York, pp 389–410.Google Scholar
  41. Huang, B., Mengersen, A., and Lee, V. D. 1988b. Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+-binding protein: Homology in its protein sequence with calmodulin and the yeast CDC31 gene product. J. Cell Biol. 107: 133–140.CrossRefGoogle Scholar
  42. Huang, B., and Pitelka, D. R. 1973. The contractile process in the ciliate Stentor coeruleus. J. Cell Biol. 57: 704–723.CrossRefGoogle Scholar
  43. Huang, B., Watterson, D. W., Lee, V. D., and Schibler, M. J. 1988a. Purification and characterization of a basal body-associated Ca2+-binding protein. J. Cell Biol. 107: 121–131.CrossRefGoogle Scholar
  44. Hyams, J. S., and Borisy, G. G. 1978. Isolated flagellar apparatus of Chlamydomonas: Characterization of forward swimming and alteration of waveform and reversal of motion by calcium ions in vitro. J. Cell Sci. 33: 235–253.Google Scholar
  45. Ishida, H., and Shigenaka, Y. 1988. Cell model contraction in the ciliate Spirostomum. Cell Motil. Cytoskel. 9: 278–282.CrossRefGoogle Scholar
  46. Jarvik, J. W., and Suhan, J. P. 1991. The role of the flagellar transition region: Inferences from the analysis of the Chlamydomonas mutant vfl-2. J. Cell Sci. (in press).Google Scholar
  47. Jones, A. R., Jahn, T. L., and Fonseca, J. R. 1970. Contraction of protoplasm. IV. Cinematographic analysis of the contraction of some peritrichs. J. Cell. Physiol. 75: 9–20.CrossRefGoogle Scholar
  48. Kater, J. M. 1929. Morphology and division of Chlamydomonas with reference to the phylogeny of the flagellar neuromotor system. Univ. Calif. Publ. Zool. 133: 125–168.Google Scholar
  49. Katsaros, C., Kreimer, G., and Melkonian, M. 1991. Localization of tubulin and a centrin-homologue in vegetative cells and developing gametangia of Ectocarpus siliculosus (Dillw.) Lyngb. (Phaeophyceae, Ectocarpales). Bot. Acta 104: 87–92.Google Scholar
  50. Koutoulis, A., McFadden, G. I., and Wetherbee, R. 1988. Spinescale reorientation in Apedinella radians (Pedinellales, Chrysophyceae): The microarchitecture and immunocytochemistry of the associated cytoskeleton. Protoplasma 147: 25–41.CrossRefGoogle Scholar
  51. Kreimer, G., and Melkonian, M. 1990. Reflection confocal laser scanning microscopy of eyespots in flagellated green algae. Eur. J. Cell Biol. 53: 101–111.Google Scholar
  52. Kristensen, B. T., Engdahl Nielsen, L., and Rostgaard, J. 1974. Variations in myoneme birefringence in relation to length changes in Stentor coeruleus. Exp. Cell Res. 85: 127–135.CrossRefGoogle Scholar
  53. Kugrens, P., and Lee, R. E. 1990. Ultrastructural evidence for bacterial incorporation and myxotrophy in the photosynthetic cryptomonad Chroomonas pochmanni Huber-Pestalozzi (Cryptomonadida). J. Protozool. 37: 263–267.Google Scholar
  54. Kugrens, P., Lee, R. E., and Andersen, R. A. 1986. Cell form and surface patterns in Chroomonas and Cryptomonas cells (Cryptophyta) as revealed by scanning electron microscopy. J. Phycol. 22: 512–522.CrossRefGoogle Scholar
  55. Lechtreck, K.-F., McFadden, G. I., Melkonian, M. 1989. The cytoskeleton of the naked green flagellate Spermatozopsis similis: Isolation, whole mount electron microscopy, and preliminary biochemical and immunological characterization. Cell Motil. Cytoskel. 14: 552–561.CrossRefGoogle Scholar
  56. Lechtreck, K.-F., and Melkonian, M. 1991. An update on fibrous flagellar roots in green algae. Protoplasma (in press).Google Scholar
  57. LeDizet, M., and Piperno, G. 1986. Cytoplasmic microtubules containing acetylated a-tubulin in Chlamydomonas reinhardtii: Spatial arrangement and properties. J. Cell Biol. 103: 13–22.CrossRefGoogle Scholar
  58. Lewin, R. A., and Burrascano, C. 1983. Another new kind of Chlamydomonas mutant, with impaired flagellar autotomy. Experientia 39: 1397–1398.CrossRefGoogle Scholar
  59. Lewin, R. A., and Lee, K. W. 1985. Autotomy of algal flagella: Electron microscope studies of Chlamydomonas (Chlorophyceae) and Tetraselmis (Prasinophyceae). Phycologia 24: 311–316.CrossRefGoogle Scholar
  60. Manton, I. 1952. The fine structure of plant cilia. Symp. Soc. Exp. Biol. 6: 306–319.Google Scholar
  61. Manton, I. 1956. Plant cilia and associated organelles. In D. Rudnick (ed.), Cellular Mechanisms in Differentiation and Growth. Princeton University Press, Princeton, pp. 61–71.Google Scholar
  62. Manton, I. 1965. Some phyletic implications of flagellar structure in plants. In R. D. Preston (ed.), Advances in Botanical Research, Vol. 2. Academic Press, London, pp. 1–34.Google Scholar
  63. Manton, I., Parke, M. 1965. Observations on the fine structure of two species of Platymonas with special reference to flagellar scales and the mode of origin of the theca. J. Mar. Biol. Assoc. U.K. 45: 743–754.CrossRefGoogle Scholar
  64. Martindale, V. E., and Salisbury, J. L. 1990. Phosphorylation of algal centrin is rapidly responsive to changes in the external milieu. J. Cell Set. 96: 395–402.Google Scholar
  65. Maruyama, T. 1981. Motion of the longitudinal flagellum in Ceratium tripos (Dinoflagellida)—a retractile flagellar motion. J. Protozool. 28: 328–336.Google Scholar
  66. Maruyama, T. 1982. Fine structure of the longitudinal flagellum in Ceratium tripos, a marine dinoflagellate. J. Cell Sci. 58: 109–123.Google Scholar
  67. Maruyama, T. 1985a. Extraction model of the longitudinal flagellum of Ceratium tripos (Dinoflagellida): Reactivation of flagellar retraction. J. Cell Sci. 75: 313–328.Google Scholar
  68. Maruyama, T. 1985b. Ionic control of the longitudinal flagellum in Ceratium tripos (Dinoflagellida). J. Protozool. 32: 196–210.Google Scholar
  69. Mattox, K. R., and Stewart, K. D. 1984. In D. E. G. Irvine and D. M. John (eds.), Systematics of the Green Algae. Academic Press, London, pp. 29–72.Google Scholar
  70. McFadden, G. I., and Melkonian, M. 1986. Golgi apparatus activity and membrane flow during scale biogenesis in the green flagellate Scherffelia dubia (Prasinophyceae). I. Flagellar regeneration. Protoplasma 130: 186–198.CrossRefGoogle Scholar
  71. McFadden, G. I., Schulze, D., Surek, B., Salisbury, J. L., and Melkonian, M. 1987. Basal body reorientation mediated by a Ca2+-modulated contractile protein. J. Cell Biol. 105: 903–912.CrossRefGoogle Scholar
  72. Melkonian, M. 1979. An ultrastructural study of the flagellate Tetraselmis cordiformis Stein (Chlorophyceae) with emphasis on the flagellar apparatus. Protoplasma 98: 139–151.CrossRefGoogle Scholar
  73. Melkonian, M. 1980a. Ultrastructural aspects of basal body associated fibrous structures in green algae: A critical review. BioSystems 12: 85–104.CrossRefGoogle Scholar
  74. Melkonian, M. 1980b. Flagellar roots, mating structure and gametic fusion in the green alga Ulva lactuca (Ulvales). J. Cell Sci. 46: 149–169.Google Scholar
  75. Melkonian, M. 1984. Flagellar apparatus ultrastructure in relation to green algal classification. In D. E. G. Irvine and D. M. John (eds.), Systematics of the Green Algae. Academic Press, London, pp. 73–120.Google Scholar
  76. Melkonian, M. 1989. Centrin-mediated motility: A novel cell motility mechanism in eukaryotic cells. Bot. Acta 102: 3–4.Google Scholar
  77. Melkonian, M. 1990. Prasinophyceae. In L. Margulis, J. O. Corliss, M. Melkonian, and D. J. Chapman (eds.), Handbook of Protoctista. Bartlett and Jones, Boston, pp. 600–607.Google Scholar
  78. Melkonian, M. 1991. Systematics and evolution of the algae. Prog. Bot. 52: 271–307.Google Scholar
  79. Melkonian, M., Becker, B., and Becker, D. 1991. Scale formation in algae. J. Electron Microsc. Tech. 17: 165–178.CrossRefGoogle Scholar
  80. Melkonian, M., McFadden, G. I., Reize, I. B., and Preisig, H. R. 1987. A light and electron microscopic study of the quadriflagellate green alga Spermatozopsis exsultans. Pl. Syst. Evol. 158: 47–61.CrossRefGoogle Scholar
  81. Melkonian, M., and Preisig, H. R. 1984. Ultrastructure of the flagellar apparatus in the green flagellate Spermatozopsis similis. Pl. Syst. Evol. 146: 145–162.CrossRefGoogle Scholar
  82. Melkonian, M., and Preisig, H. R. 1986. A light and electron microscopic study of Scherffelia dubia, a new member of the scaly green flagellates (Prasinophyceae). Nord. J. Bot. 6: 235–256.CrossRefGoogle Scholar
  83. Melkonian, M., Schulze, D., McFadden, G. I., and Robenek, H., 1988. A polyclonal antibody (anti-centrin) distinguishes between two types of fibrous flagellar roots in green algae. Protoplasma 144: 56–61.CrossRefGoogle Scholar
  84. Metzner, P. 1929. Bewegungsstudien an Peridineen. Z. Bot. 22: 225–265.Google Scholar
  85. Mignot, J.-P., Joyon, L., and Pringsheim, E. G. 1968. Complements a l’etude cytologique des cryptomonadines. Protistologica 4: 493–506.Google Scholar
  86. Moestrup., Ø. 1978. On the phylogenetic validity of the flagellar apparatus in green algae and other chlorophyll a and b containing plants. BioSystems 10: 117–144.Google Scholar
  87. Moestrup, Ø. 1982. Flagellar structure in algae: A review, with new observations particularly on the Chrysophyceae, Phaeophyceae (Fucophyceae), Euglenophyceae, and Reckertia. Phycologia 21: 427–528.CrossRefGoogle Scholar
  88. Moestrup, Ø. and Andersen, R. A. 1991. Organization of heterotrophic heterokonts. In D. J. Patterson and J. Larsen (eds.), The Biology of Free-Living Heterotrophic Flagellates, Clarendon Press, Oxford, in press.Google Scholar
  89. Moncrief, N. D., Kretsinger, R. H. and Goodman, M. 1990. Evolution of EF-hand calcium-modulated proteins. I. Relationships based on amino acid sequences. J. Mol. Evol. 30: 522–562.CrossRefGoogle Scholar
  90. O’Kelly, C. J., and Floyd, G. L. 1984. Flagellar apparatus absolute orientations and the phylogeny of the green algae. BioSystems 16: 227–252.CrossRefGoogle Scholar
  91. Parke, M., and Manton, I. 1965. Preliminary observations on the fine structure of Prasinocladus marinus. J. Mar. Biol. Assoc. U.K. 45: 525–536.CrossRefGoogle Scholar
  92. Peters, H. 1929. Über Orts- und Geisselbewegung bei marinen Dinoflagellaten. Arch. Protistenkd. 67: 291–321.Google Scholar
  93. Pickett-Heaps, J. D. 1971. Reproduction by zoospores in Oedogonium. I. Zoosporogenesis. Protoplasma 72: 275–314.CrossRefGoogle Scholar
  94. Pickett-Heaps, J. D. 1975. Green Algae: Structure, Reproduction and Evolution in Selected Genera. Sinauer, Sunderland, MA, 605 pp.Google Scholar
  95. Piperno, G., and Ramanis, Z. 1991. The proximal portion of Chlamydomonas flagella contains a distinct set of inner dynein arms. J. Cell Biol. 112: 701–709.CrossRefGoogle Scholar
  96. Piperno, G., Ramanis, Z., Smith, E. F. and Sale, W. S. 1990. Three distinct inner dynein arms in Chlamydomonas flagellar Molecular composition and location in the axoneme. J. Cell Biol. 110: 379–389.CrossRefGoogle Scholar
  97. Pitelka, D. R. 1969. Fibrillar systems in protozoa. Res. Protozool. 3: 279–388.Google Scholar
  98. Preisig, H. R., and Melkonian, M. 1984. A light and electron microscopical study of the green flagellate Spermatozopsis similis sp. nov. Pl. Syst. Evol. 146: 57–74.CrossRefGoogle Scholar
  99. Randall, J. T., and Jackson, S. F. 1958. Fine structure and function in Stentor polymorphus. J. Biophys. Biocbem. Cytol. 4: 807–830.CrossRefGoogle Scholar
  100. Reize, I. B., and Melkonian, M. 1987. Flagellar regeneration in the scaly green flagellate Tetraselmis striata (Prasinophyceae): Regeneration kinetics and effect of inhibitors. Helgol. wiss. Meeresunters. 41: 149–164.CrossRefGoogle Scholar
  101. Reize, I. B., and Melkonian, M. 1989. A new way to investigate living flagellated/ ciliated cells in the light microscope: Immobilization of cells in agarose. Bot. Acta 102: 145–151.Google Scholar
  102. Ricketts, T. R. 1979. The induction of synchronous cell division in Platymonas striata Butcher (Prasinophyceae). Br. phycol. J. 14: 219–223.CrossRefGoogle Scholar
  103. Ringo, D. L. 1967. Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas reinhardtii. J. Cell Biol. 33: 543–571.CrossRefGoogle Scholar
  104. Robenek, H., and Melkonian, M. 1979. Rhizoplast-membrane associations in the flagellate Tetraselmis cordiformis Stein (Chlorophyceae) revealed by freeze-fracture and thin sections. Arch. Protistenkd. 122: 340–351.CrossRefGoogle Scholar
  105. Roberts, K. R. 1984. Structure and significance of the cryptomonad flagellar apparatus. I. Cryptomonas ovata (Cryptophyta). J. Phycol. 20: 590–599.CrossRefGoogle Scholar
  106. Roberts, K. R., and Roberts, J. E. 1991. The flagellar apparatus and cytoskeleton of the Dinoflagellates: a comparative overview. Protoplasma (in press).Google Scholar
  107. Roberts, K. R., Stewart, K. D., and Mattox, K. R. 1981. The flagellar apparatus of Chilomonas Paramecium and its comparison with certain zooflagellates. J. Phycol. 17: 159–167.CrossRefGoogle Scholar
  108. Routledge, L. M. 1978. Calcium-binding proteins in the vorticellid spasmoneme. J. Cell Biol. 77: 358–370.CrossRefGoogle Scholar
  109. Routledge, L. M., Amos, W. B., Gupta, B. L., Hall, T. A. and Weis-Fogh, T. 1975. Microprobe measurements of calcium-binding in the contractile spasmoneme of a vorticellid. J. Cell Sci. 19: 195–201.Google Scholar
  110. Routledge, L. M., Amos, W. B., Yew, F. F. and Weis-Fogh, T. 1976. New calcium-binding contractile proteins. In R. Goldman, T. Pollard, and J. Rosenbaum (eds.), Cell Motility, Cold Spring Harbor Laboratory, pp. 93–113.Google Scholar
  111. Riiffer, U., and Nultsch, W. 1985. High-speed cinematographic analysis of the movement of Chlamydomonas. Cell Motil. 5: 251–263.CrossRefGoogle Scholar
  112. Sabatini, D. D., Bensch, K., and Barnett, R. J. 1963. Cytochemistry and electron microscopy. J. Cell Biol. 17: 19–58.CrossRefGoogle Scholar
  113. Salisbury, J. L. 1982. Calcium-sequestering vesicles and contractile flagellar roots. J. Cell Sci. 58: 433–443.Google Scholar
  114. Salisbury, J. L. 1983. Contractile flagellar roots: The role of calcium. J. Submicrosc. Cytol. 15: 105–110.Google Scholar
  115. Salisbury, J. L. 1989a. Algal centrin-calcium-sensitive contractile organelles. In A. W. Coleman, L. J. Goff, and J. R. Stein-Taylor (eds.), Algae as Experimental Systems, Alan R. Liss, New York, pp. 19–37.Google Scholar
  116. Salisbury, J. L. 1989b. Centrin and the algal flagellar apparatus. J. Phycol. 25: 201–206.CrossRefGoogle Scholar
  117. Salisbury, J. L., Baron, A., Surek, B., and Melkonian, M. 1984. Striated flagellar roots: Isolation and partial characterization on a calcium-modulated contractile organelle. J. Cell Biol. 99: 962–970.CrossRefGoogle Scholar
  118. Salisbury, J. L., Baron, A. T., Coling, D. E., Martindale, V. E., and Sanders, M. A. 1986. Calcium-modulated contractile proteins associated with the eucaryotic centrosome. Cell Motil. 6: 193–197.CrossRefGoogle Scholar
  119. Salisbury, J. L., Baron, A. T., and Sanders, M. A. 1988. The centrin-based cytoskeleton of Chlamydomonas reinhardtii: Distribution in interphase and mitotic cells. J. Cell Biol. 107: 635–641.CrossRefGoogle Scholar
  120. Salisbury, J. L., and Floyd, G. L. 1978. Calcium-induced contraction of the rhizoplast of a quadriflagellate green alga. Science 202: 975–977.CrossRefGoogle Scholar
  121. Salisbury, J. L., and Greenwood, T. M. 1990. Molecular conservation of the eucaryotic centrosome: Assembly of algal centrin into functional vertebrate centrosomes. J. Cell Biol. 111: 181a.Google Scholar
  122. Salisbury, J. L., Sanders, M. A., and Harpst, L. 1987. Flagellar root contraction and nuclear movement during flagellar regeneration in Chlamydomonas reinhardtii. J. Cell Biol. 105: 1799–1805.CrossRefGoogle Scholar
  123. Salisbury, J. L., Swanson, J. A., Floyd, G. L., Hall, R., and Maihle, N. J. 1981. Ultrastructure of the flagellar apparatus of the green alga Tetraselmis subcordiformis with special consideration given to the function of the rhizoplast and rhizanchora. Protoplasma 107: 1–11.CrossRefGoogle Scholar
  124. Sanders, M. A., and J. L. Salisbury. 1989. Centrin-mediated microtubule severing during flagellar excision in Chlamydomonas reinhardtii. J. Cell Biol. 108: 1751–1760.CrossRefGoogle Scholar
  125. Schlosser, U. G. 1982. Sammlung von Algenkulturen. Ber. Deutsch. Bot. Ges. 95: 181–276.Google Scholar
  126. Schnepf, E., and Maiwald, M. 1970. Halbdesmosomen bei Phytoflagellaten. Experientia 26: 1343.CrossRefGoogle Scholar
  127. Schulze, D., Robenek, H., McFadden, G. I., and Melkonian, M. 1987. Immunolocalization of a Ca2+-modulated contractile protein in the flagellar apparatus of green algae: the nucleus-basal body connector. Eur. J. Cell Biol. 45: 51–61.Google Scholar
  128. Schütt, F. 1895. Die Peridineen der Plankton-Expedition. I. Studien über die Zellen der Peridineen. Ergebn. Plankton-Exped. (Kiel und Leipzig) 4: 1–170.Google Scholar
  129. Stewart, K. D., and Mattox, K. R. 1975. Comparative cytology, evolution and classification of the green algae with some consideration of the origin of other organisms with chlorophyll a and b. Bot. Rev. 41: 104–135.CrossRefGoogle Scholar
  130. van Leeuwenhoek, A. 1676. Letter. Phil. Trans. Roy. Soc. Lond. B 12: 133.Google Scholar
  131. Weis-Fogh, T., and Amos, W. B. 1972. Evidence for a new mechanism of cell motility. Nature 236: 301–304.CrossRefGoogle Scholar
  132. Wetherbee, R., and Andersen, R. A. 1991. Flagella of chrysophycean algae play an active role in prey capture and selection: direct observations on Epipyxis pulchra and Ochromonas danica using image enhanced video microscopy. Cell Motil. Cytoskel. (submitted).Google Scholar
  133. White, R. B., and Brown, D. L. 1981. ATPase activities associated with the flagellar basal apparatus of Polytomella. J. Ultrastruct. Res. 75: 151–161.CrossRefGoogle Scholar
  134. Wright, R. L., Salisbury, J., and Jarvik, J. W. 1985. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation. J. Cell Biol. 101: 1903–1912.CrossRefGoogle Scholar
  135. Wright, R. L., Adler, S. A., Spanier, J. G., and Jarvik, J. W. 1989. Nucleus-basal body connector in Chlamydomonas: Evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Cell Motil. Cytoskel. 14: 516–526.CrossRefGoogle Scholar
  136. Yogosawa-Ohara, R., Sizaki, T., and Shigenaka, Y. 1985. Twisting contraction mechanism of a heterotrichous ciliate, Spirostomum ambiguum. 2. Role of longitudinal micro tubular sheet. Cytobios 44: 215–230.Google Scholar
  137. Zimmermann, W. 1925. Helgolander Meeresalgen I-VI. Wiss. Meeresunters. Abt. Helgoland 16: 1–25.Google Scholar

Copyright information

© Routledge, Chapman & Hall, Inc. 1992

Authors and Affiliations

  • Michael Melkonian
  • Peter L. Beech
  • Christos Katsaros
  • Dorothee Schulze

There are no affiliations available

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