Structural Characterization of IBV Glycoproteins
Avian infectious bronchitis virus (IBV) causes economically important disease in chickens, affecting both respiratory and non-respiratory tissues. Like other coronaviruses IBV virions contain three virus-coded protein structures, the spike (S; surface projection, peplomer), nucleocapsid (N) and matrix (M) proteins1. The polypeptides of M and N proteins have been identified. The M protein comprises a polypeptide of mol. wt. 23 000 (23K) which is glycosylated to different extents, the major glycopolypeptide being about 30K2,3,4. A polypeptide of about 50K forms the N protein5. However, although many papers have been published on the composition of IBV, there has been a distinct lack of agreement on the number and mol. wt. of the polypeptides which form the spikes (for references see1,6.
KeywordsSucrose Gradient Disulphide Bond Infectious Bronchitis Virus Semliki Forest Virus High Mannose
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- 3.D. F, Stem and B. M. Sefton. Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins. J. Virol. 44: 804 (1982).Google Scholar
- 6.D. Cavanagh. Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides. J. Gen. Virol. 64: in press (1983).Google Scholar
- 16.R. Eason and A. M. Campbell. Analytical centrifugation, in“Centrifugal Separation in Molecular Biology and Cell Biology” G. D. Birnie and D. Rickwood, eds., Butterworths, London. (1978).Google Scholar