In Vitro Assembly of the Murine Coronavirus Membrane Protein E1

  • Peter Rottier
  • Dorothée Brandenburg
  • John Armstrong
  • Ben van der Zeijst
  • Graham Warren
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)


One of the most striking differences between coronaviruses and other enveloped RNA viruses is that they bud into intracellular membranes. Only bunyaviruses behave similarly (1). Coronavirions have been observed in the lumens of endoplasmic reticulum (ER) and Golgi apparatus from cells infected with various coronaviruses such as mouse hepatitis virus (MHV) (2–5), avian infectious bronchitis virus (IBV) (6) and porcine enteric Coronavirus (7) The assembled virions then appear in smooth-walled vesicles en route to the plasma membrane (PM) where they are probably released from the cell by exocytosis (4,8).


Infectious Bronchitis Virus Endoplasmic Reticulum Membrane Microsomal Membrane Protease Digestion Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    J.F. Smith and D,Y. Pifat, Virology 121:61 (1982).PubMedCrossRefGoogle Scholar
  2. 2.
    A. Massalski, M. Coulter-Mackie and S. Dales, in: “Biochemistry and Biology of Coronaviruses”, V. ter Meulen, S. Siddell and H. Wege, eds., Plenum Press, New York and London, pp. 111–118 (1981).Google Scholar
  3. 3.
    K.V.Holmes, E.W. Doller and L.S.Sturman, Virology 115:334 (1981).PubMedCrossRefGoogle Scholar
  4. 4.
    M.E. Dubois-Dalcq, E.W. Doller, M.V. Haspel and K.V. Holmes, Virology 119:317 (1982).PubMedCrossRefGoogle Scholar
  5. 5.
    H.Niemann, B. Boschek, D. Evans, M. Rosing, T. Tamura and H.-D. Klenk, EMBO J. 1:1499 (1982).PubMedGoogle Scholar
  6. 6.
    D.Chasey and D.J.Alexander, Arch.Virol. 52:101 (1976).PubMedCrossRefGoogle Scholar
  7. 7.
    R. Ducatelle, W. Coussement, M.B. Pensaert, P. DeBouck and J.Hoorens, Arch.Virol. 68:35 (1981).PubMedCrossRefGoogle Scholar
  8. 8.
    K.V. Holmes and J.N. Behnke, in: “Biochemistry and Biology of Coronaviruses”, V. ter Meulen, S. Siddell and H. Wege, eds.. Plenum Press, New York and London, pp. 287–299 (1981).Google Scholar
  9. 9.
    P.J.M.Rottier, M.C.Horzinek and B.A.M. van der Zeijst, J.Virol. 40:350 (1981).PubMedGoogle Scholar
  10. 10.
    H.Niemann, and H.-D. Klenk, J.Mol.Biol. 153:993 (1981).PubMedCrossRefGoogle Scholar
  11. 11.
    D.C. Johnson and P.G. Spear, Cell 32:987 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    W.J.M. Spaan, P.J.M. Rottier, M.C. Horzinek and B.A.M. van der Zeijst, Virology 108:424 (1981).PubMedCrossRefGoogle Scholar
  13. 13.
    P.J.M. Rottier, W.J.M. Spaan, M.C. Horzinek and B.A.M. van der Zeijst, J.Virol. 38:20 (1981).PubMedGoogle Scholar
  14. 14.
    B.A.M. van der Zeijst, B.E. Noyes, M.-E. Mirault, B. Parker, A.D.M.E. Osterhaus, F.A. Swyryd, N. Bleumink, M.C. Horzinek and G.R. Stark, J.Virol., in press (1983).Google Scholar
  15. 15.
    F.H. Wilt, Cell 11:673 (1977).PubMedCrossRefGoogle Scholar
  16. 16.
    H.R.B. Pelham and R.J.Jackson, Eur.J. Biochem. 67:247 (1976).PubMedCrossRefGoogle Scholar
  17. 17.
    G. Blobel and B. Bobberstein, J.Cell Biol. 67:852 (1975).PubMedCrossRefGoogle Scholar
  18. 18.
    H. Garoff, K. Simons and B.Bobberstein, J.Mol.Biol. 124:587 (1978).PubMedCrossRefGoogle Scholar
  19. 19.
    W.M. Stanley, Anal.Biochem. 48:202 (1972).PubMedCrossRefGoogle Scholar
  20. 20.
    J. Green, G. Griffiths, D. Louvard, P. Quinn and G. Warren, J.Mol.Biol. 152:663 (1981).PubMedCrossRefGoogle Scholar
  21. 21.
    C. Bordier, J.Biol.Chem. 256:1604 (1981).PubMedGoogle Scholar
  22. 22.
    L.S. Sturman, Virology 77:637 (1977).PubMedCrossRefGoogle Scholar
  23. 23.
    D.D. Sabatini and G. Blobel, J.Cell Biol. 45:146 (1970).PubMedCrossRefGoogle Scholar
  24. 24.
    T.Obrig, J. Irvin, W. Culp and B. Hardesty, Eur.J.Biochem. 21:31 (1971).PubMedCrossRefGoogle Scholar
  25. 25.
    R.Henderson and P.N.T.Unwin, Nature 257:28 (1975).PubMedCrossRefGoogle Scholar
  26. 26.
    F.A.Dratz and P.A.Hargrave, Trends in Biochem.Sci. 8:128 (1983).CrossRefGoogle Scholar
  27. 27.
    T.L. Steck, J.Supramol.Struc. 8:311 (1978).CrossRefGoogle Scholar
  28. 28.
    M. Noda, H. Takahashi, T. Tanabe, M. Toyosato, S. Kikyotani, Y. Furutani, T. Hirose, H. Takashima, S. Inayama, T, Miyata and S. Numa, Nature 302:528 (1983).PubMedCrossRefGoogle Scholar
  29. 29.
    Yu.A. Ovchinnikov, N.G. Abdulaev, M.Yu. Feigina, A.V. Kiselev, N.A. Lobanov, FEBS Lett. 100: 219 (1979).PubMedCrossRefGoogle Scholar
  30. 30.
    I. Schechter, Y. Burstein, R. Zemell, E. Ziv, F. Kantor and D.S. Papermaster, Proc.Natl.Acad.Sci. USA 76:2654 (1979).PubMedCrossRefGoogle Scholar
  31. 31.
    W.A. Braell and H.F. Lodish, Cell 28:23 (1982).PubMedCrossRefGoogle Scholar
  32. 32.
    R.D. Palmiter, J.Gagnon and K.A. Walsh, Proc.Natl.Acad.Sci. USA 75:94 (1978).PubMedCrossRefGoogle Scholar
  33. 33.
    V.R. Lingappa, J.R. Lingappa and G. Blobel, Nature 281:117 (1979).PubMedCrossRefGoogle Scholar
  34. 34.
    R.D. Meek, K.A. Walsh and R.D. Palmiter, J. Biol. Chem. 257:12245 (1982).PubMedGoogle Scholar
  35. 35.
    D.F. Stern and B.M. Sefton, J.Virol. 44:804 (1982).PubMedGoogle Scholar
  36. 36.
    H. Shida and S. Dales, Virology 111:56 (1981).PubMedCrossRefGoogle Scholar
  37. 37.
    L.S. Sturman, K.V. Holmes and J. Behnke, J.Virol. 33:449 (1980).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Peter Rottier
    • 1
  • Dorothée Brandenburg
    • 1
  • John Armstrong
    • 2
  • Ben van der Zeijst
    • 1
  • Graham Warren
    • 2
  1. 1.Institute of Virology, Veterinary FacultyState UniversityUtrechtThe Netherlands
  2. 2.European Molecular Biology Laboratory69 HeidelbergGermany

Personalised recommendations