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Characterization of MHV-A59 Persistently Infected Cells

  • Reinald Repp
  • Teruko Tamura
  • Hans-Dieter Klenk
  • Heiner Niemann
Chapter
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Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)

Abstract

The aim of our work is to characterize MHV-A59 persistently infected cloned 17C11 cell lines on a molecular level. These cell lines were obtained by three consecutive infections of 17C11 cells (2 PFU/cell) and cloned three times by limited dilution in the presence of conditioned medium which contained 30 percent ultrafiltrated supernatant from 17C11 cells.

Keywords

Conditioned Medium 17C11 Cell Viral cDNA Limited Dilution Fusion Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Reinald Repp
    • 1
  • Teruko Tamura
    • 1
  • Hans-Dieter Klenk
    • 1
  • Heiner Niemann
    • 1
  1. 1.Institut fuer Virologie, Fachbereich HumanmedizinJustus-Liebig-Universitaet GiessenGiessenGermany

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