Nucleotide Sequencing of Mouse Hepatitis Virus Strain JHM Messenger RNA 7
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Determination of the nucleic acid sequence of MHV genomic and subgenomic RNAs is clearly a way of predicting the primary structure of encoded polypeptides and at the same time identifying non-coding regions, which may be relevant to the regulation of the viral genes and to the replication of the viral RNA. A comparative analysis of the sequences of different MHV strains, or mutants with altered phenotypes, and of other coronaviruses will also be a method of identifying functionally important regions by their conserved sequences. Such analysis will also be required to relate changes in nucleotide sequence to changes, for example, in host range and pathogenicity.
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