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Electron Lucent Structures Induced by Coronaviruses

  • M. C. Kemp
  • A. Harrison
  • J. C. Hierholzer
  • J. S. Burks
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 173)

Abstract

Viral inclusion bodies have long been considered pathognomoic. Coronaviruses cause the formation of cytoplasmic inclusions consisting of densely staining particles contained within a granular matrix enclosed by a double membrane (1). During studies on the assembly of 229E in human embryomic lung fibroblasts roughly circular electron lucent structures with dense limiting membranes, sometimes empty but frequently containing net-like beaded strands, were seen in the cytoplasm of infected cells that differed from the previously described inclusions. These structures vary in size from 300–900nm in diameter (Fig. 1) and appear at approximately 6hr post-infection (PI), i.e. at a time when viral protein synthesis is initiated (2) and before the assembly of virions (3).

Keywords

Multiple Sclerosis Semliki Forest Virus Human Embryonic Lung Viral Protein Synthesis Granular Matrix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    L.S. Oshiro, J.H. Schieble, and E.H. Lennette, Electron Microscopie Studies of Coronavirus, J. Gen Virol. 12:161(1971).PubMedCrossRefGoogle Scholar
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    M.C. Kemp, J.C. Hierholzer, A. Harrison, and J.S. Burks, Characterization of Viral Proteins Synthesized in 229E Infected Cells and Effect(s) of Inhibition of Glycosylcation and Glycoprotein Transport on This Process (See these proceedings).Google Scholar
  3. 3.
    M.C. Kemp, A. Harrison, J.C. Hierholzer, and J.S. Burks, Assembly of 229E Virions in Human Embryonic Lung Fibroblasts and Effects of Inhibition of Glycosylation and Glycoprotein Transport on This Process (See these proceedings).Google Scholar
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    J.S. Burks, B. L. DeVald, L.D. Jankovsky, and J.C. Gerdes, Two Coronaviruses Isolated from Central Nervous System of Two Multiple Sclerosis Patients, Science 209:933 (1980).PubMedCrossRefGoogle Scholar
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    P.M. Grimley, I. K. Berezesky, and R.M. Friedman, Cytoplasmic Structures Associated with an Arbovirus Infection: Loci of Viral Ribonucleic Acid Synthesis, J. Virol. 2:1326 (1968).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • M. C. Kemp
    • 1
    • 2
    • 3
  • A. Harrison
    • 4
  • J. C. Hierholzer
    • 4
  • J. S. Burks
    • 1
    • 2
    • 3
  1. 1.Center for Neurological DiseasesRocky Mountain Multiple Sclerosis CenterUSA
  2. 2.Veterans Administration Medical CenterUSA
  3. 3.University of Colorado School of MedicineDenverUSA
  4. 4.Division of Viral DiseasesCenters for Disease ControlAtlantaUSA

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