Electron Lucent Structures Induced by Coronaviruses
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Viral inclusion bodies have long been considered pathognomoic. Coronaviruses cause the formation of cytoplasmic inclusions consisting of densely staining particles contained within a granular matrix enclosed by a double membrane (1). During studies on the assembly of 229E in human embryomic lung fibroblasts roughly circular electron lucent structures with dense limiting membranes, sometimes empty but frequently containing net-like beaded strands, were seen in the cytoplasm of infected cells that differed from the previously described inclusions. These structures vary in size from 300–900nm in diameter (Fig. 1) and appear at approximately 6hr post-infection (PI), i.e. at a time when viral protein synthesis is initiated (2) and before the assembly of virions (3).
KeywordsMultiple Sclerosis Semliki Forest Virus Human Embryonic Lung Viral Protein Synthesis Granular Matrix
- 2.M.C. Kemp, J.C. Hierholzer, A. Harrison, and J.S. Burks, Characterization of Viral Proteins Synthesized in 229E Infected Cells and Effect(s) of Inhibition of Glycosylcation and Glycoprotein Transport on This Process (See these proceedings).Google Scholar
- 3.M.C. Kemp, A. Harrison, J.C. Hierholzer, and J.S. Burks, Assembly of 229E Virions in Human Embryonic Lung Fibroblasts and Effects of Inhibition of Glycosylation and Glycoprotein Transport on This Process (See these proceedings).Google Scholar