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The Detection of Chemical Mutagens with Enteric Bacteria

  • Bruce N. Ames

Abstract

Mutagens alter DNA. As the DNA of all organisms has the same double helical structure and the same four nucleotides, any organism may be used as an indicator system for mutagens. Bacteria have numerous advantages for the detection of mutagens, and we discuss these and describe various methods and strains. We also discuss the validity of the bacterial tests and their pertinence to human mutagenesis and carcinogenesis.

Keywords

Petri Plate Enteric Bacterium Nitrogen Mustard Chemical Mutagen Nitrate Reductase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ames, B. N., and Whitfield, H. J. (1966), Cold Spring Harbor Symp. Quant. Biol. 31, 221.PubMedCrossRefGoogle Scholar
  2. Demerec, M., Bertani, G., and Flint, J. (1951), Am. Naturalist 85, 119.CrossRefGoogle Scholar
  3. Fukusawa, T., and Nikaido, H. (1961), Genetics 46, 1295.Google Scholar
  4. Gorini, L., and Kataja, E., Biochem. Biophys. Res. Commun. (1965) 18, 656.CrossRefGoogle Scholar
  5. Heinemann, B., Howard, A. J., and Hollister, Z. J. (1967), Appl. Microbiol. 15, 723.PubMedGoogle Scholar
  6. Hirota, Y. (1960), Proc. Nat. Acad. Sci. 46, 57.PubMedCrossRefGoogle Scholar
  7. Iyer, V. N., and Szybalski, W. (1958), Appl. Microbiol. 6, 23.PubMedGoogle Scholar
  8. Oeschger, N. S., and Hartman, P. E. (1970), J. Bacteriol. 101, 490.PubMedGoogle Scholar
  9. Sander, J., Schweinsberg, F., and Menz, H.-P. (1968), Hoppe-Seylers Z. Physiol. Chem. 349, 1691.PubMedCrossRefGoogle Scholar
  10. Schwartz, D. O., and Beckwith, J. R. (1969), Genetics 61, 371.PubMedGoogle Scholar
  11. Sideropoulos, A. S., and Shankel, D. M. (1968), J. Bacteriol. 96, 198.PubMedGoogle Scholar
  12. Silengo, L., Schlessinger, D., Mangiarotti, G., and Apirion, D. (1967), Mutation Res. 4, 701.PubMedCrossRefGoogle Scholar
  13. Smith, D. W. E. (1966), Science 152, 1273.PubMedCrossRefGoogle Scholar
  14. Spatz, M., Dougherty, W. J., and Smith, D. W. E. (1967), Proc. Soc. Exp. Biol. Med. 124, 476.PubMedGoogle Scholar
  15. Szybalski, W. (1958), N. Y. Acad. Sci. Proc. 76, 475.Google Scholar
  16. Vogel, H. J., and Bonner, D. M. (1956), J. Biol. Chem. 218, 97.PubMedGoogle Scholar
  17. Whitfield, H. J., Martin, R. G., and Ames, B. N. (1966), J. Mol. Biol. 21, 335.PubMedCrossRefGoogle Scholar
  18. Witkin, E. (1969), Ann. Rev. Microbiol. 23, 437.CrossRefGoogle Scholar
  19. Yourno, J., and Heath, S. (1969), J. Bacteriol. 100, 460.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1971

Authors and Affiliations

  • Bruce N. Ames
    • 1
  1. 1.Biochemistry DepartmentUniversity of CaliforniaBerkeleyUSA

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