Culture of Phytoplankton for Feeding Marine Invertebrates

  • Robert R. L. Guillard


These pages describe relatively simple and reliable methods for the culture of marine phytoplankton species useful for feeding marine invertebrates. The methods suffice for the most fastidious algae now routinely cultivable, and simplifications indicated for less demanding species are easily made; for example, omission of silicate for plants other than diatoms. Certain modifications of techniques, ancillary methods, and precautions will be treated briefly because questions often arise concerning them, but documentation will be minimal and hopefully restricted to publications readily available.


Trace Metal Stock Culture Algal Culture Artificial Seawater Trace Metal Solution 
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  1. Clark, R.C., Jr. 1966. Saturated hydrocarbons in marine plants and sediments, M.S, Thesis, Massachusetts Institute of Technology, June 1966, (Dept, of Geology and Geophysics), 96 pp.Google Scholar
  2. Droop, M.R. 1961, Some chemical considerations in the design of synthetic media for marine algae, Botanica Marina II, Fasc, 3/4, 231–246CrossRefGoogle Scholar
  3. Fogg, G.E. 1965. Algal Cultures and Phytoplankton Ecology. University of Wisconsin Press, 126 ppGoogle Scholar
  4. Gerloff, GC., GP, Fitzgerald, and F, Skoog., 1950. The isolation, purification and nutrient solution requirements of blue-green algae, pp. 27–44, In: The Culturing of Algae, C.F, Kettering Foundation, J. Brunei, GW, Prescott, and LH. Tiffany, Editors, 114 pp.Google Scholar
  5. Gross, F, 1932, Notes on the culture of some marine plankton organisms, J. Mar, Biol, Assoc, U.K, 21: 753–768Google Scholar
  6. Guillard, R.R.L, 1943. Organic sources of nitrogen for marine centric diatoms, Ch, 9 pp, 93–104. In: Symposium on Maxine Microbiology, C.H. Oppenheimer, Ed. C.C. Thomas, Springfield, Illinois. 769 pp.Google Scholar
  7. Guillard, R.R.L. and J.H. Ryther., 1962. Studies on marine planktonic diatoms I. Cyclotella nana Hustedt and Detonula confervacae (Cleve) Gran. Can. J. Microbiol. 8: 229–239PubMedCrossRefGoogle Scholar
  8. Harvey, H.W. 1955, The Chemistry and Fertility of Sea Waters, Cambridge University Press, 224 pp.Google Scholar
  9. Hutchinson, G.E. 1957, A Treatis on Limnology, Vol. I, John Wiley, N.Y. 1015 pp.Google Scholar
  10. Jfrgensen, C.B. 1966. Biology of Suspension Feeders. Pergamon Press. 357 pp.Google Scholar
  11. Maddux, W.S. 1966. A 4p light meter. Limnol. Oceanogr. 11: 136- 137.CrossRefGoogle Scholar
  12. McLachlan, J. 1964. Some considerations of the growth of marine algae in artificial media. Can. J. Microbiol. 10: 769.782.Google Scholar
  13. Morris, I. 1967. An Introduction to the Algae. Hutchinson University Library, London. 189 PP.Google Scholar
  14. Meyers, J, 1962. Laboratory cultures, 39, In: Physiology and Biochemistry of Algae, R.A. Lewin, Ed, Academic Press, 929 pp.Google Scholar
  15. Provasoli, L, and IJ, Pintner, 1960. Artificial media for freshwater algae: problems and suggestions, 84–96, In: The Ecology of Algae, Special Pub, No, 2, Pymatuning Laboratory of Field Biology, University Pittsburgh, GA, Tryon Jr, and RT, Hartman, Eds. 96 pp.Google Scholar
  16. Provasoli, L., JJA, McLaughlin and M. Droop. 1957. The development of artificial media for marine algae. Arch, Mikrobiol, 25: 392–428.CrossRefGoogle Scholar
  17. Siegelman, H.W. and R.R.L. Guillard. 1971. Large-scale culture of algae. Methods in Enzymology 23: 110–115.CrossRefGoogle Scholar
  18. Spencer, G.P. 1966. Theoretical aspects of the control of pH in natural seawater and synthetic culture media for marine algae. Botanica Marine 9: 81–99.CrossRefGoogle Scholar
  19. Stein, J.R. 1973. Handbook of Phycological Methods. Cambridge University Press, 448 pp.Google Scholar
  20. Ukeles, R. 1971. Nutritional requirements in shellfish culture. In: Proceedings of the Conference on Artificial Propagation of Commercially Valuable Shellfish, Oct. 22–23, 1969. College of Marine Studies, University of Delaware, Newark, Delaware.Google Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Robert R. L. Guillard
    • 1
  1. 1.Biology DepartmentWoods Hole Oceanographic InstitutionWoods HoleUSA

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