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Science and Technology of Patch-Recording Electrodes

  • D. P. Corey
  • C. F. Stevens

Abstract

Patch recording, in its present form, was made possible by the discovery that under some circumstances the membrane of a cell can seal extraordinarily tightly to the tip of a glass microelectrode. Because the seal is electrically tight, electrical noise is reduced by several orders of magnitude. Because the glass-membrane bond is mechanically strong, the patch of membrane spanning the tip may be isolated from the cell, or the patch may be ruptured to create access to the inside of the cell. This chapter deals with the properties of glasses and cell membranes that make such a seal occur (the “science”) and describes in detail the construction of micropipettes for patch recording (the “ technology”).

Keywords

Silicon Atom Quartz Glass Fuse Quartz Sodium Oxide Boric Oxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hay, E. D., 1981, Extracellular matrix, J. Cell Biol. 91:205s–223s.PubMedCrossRefGoogle Scholar
  2. Warren, B. E., 1940, X-ray diffraction study of the structure of glass, Chem. Rev. 26:237–255.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • D. P. Corey
    • 1
  • C. F. Stevens
    • 1
  1. 1.Section of Molecular NeurobiologyYale University Medical SchoolNew HavenUSA

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