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Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes

  • Otto Z. Sellinger
  • Julio M. Azcurra

Abstract

Since the appearance of the procedure of Norton and Poduslo (1970), reviewed in detail in the first volume of this series, several new procedures for the bulk separation of neuronal cell bodies and glial cells have been reported in which all steps are carried out in the cold and no digestive enzyme(s) (Norton and Poduslo, 1970; Hemminki and Holmila, 1971; Giorgi, 1971; Kuenzle et al., 1972) is used to facilitate cell separation. In this chapter, we give a detailed description of these procedures and we also present a general survey of their applications in cellular neurochemistry.

Keywords

Glial Cell Cell Body Neuronal Cell Body Nerve Cell Body Microsomal Pellet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Otto Z. Sellinger
    • 1
  • Julio M. Azcurra
    • 1
  1. 1.Laboratory of Neurochemistry, Mental Health Research InstituteUniversity of Michigan Medical CenterAnn ArborUSA

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