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Fluorescence-Based Automated DNA Sequence Analysis

  • Lloyd M. Smith
Part of the Genetic Engineering book series (GEPM, volume 10)

Abstract

DNA sequence analysis is one of the most important techniques of modern molecular biology. It permits the rapid and complete determination of the precise chemical structure of the genetic material. This information is the foundation for the subsequent study of genes and their gene products. Such work includes the analysis of gene expression, protein structure and function, and the evolution, polymorphism, and diversity of the genome.

Keywords

Enzymatic Sequencing Multicomponent Analysis Modern Molecular Biology Data Point Number Enzymatic Sequencing Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Sanger, F., Nicklen, S. and Coulson, A.R. (1977) Proc. Nat. Acad. Sci. U.S.A. 74, 5463–5467.Google Scholar
  2. 2.
    Maxam, A.M. and Gilbert, W. (1977) Proc. Nat. Acad. Sci. U.S.A. 74, 560–564.Google Scholar
  3. 3.
    Smith, L.M., Fung, S., Hunkapiller, M.W., Hunkapiller, T.J. and Hood, L.E. (1985) Nucl. Acids Res. 13, 2399–2412.PubMedCrossRefGoogle Scholar
  4. 4.
    Smith, L.M., Sanders, J.Z., Kaiser, R.J., Hughes, P., Dodd, C., Connell, C.R., Heiner, C., Kent, S.B.H. and Hood, L.E. (1986) Nature 321, 674–679.PubMedCrossRefGoogle Scholar
  5. 5.
    Beaucage, S.L. and Caruthers, M.H. (1981) Tet. Lett. 22 (20), 1859–1862.CrossRefGoogle Scholar
  6. 6.
    Connell, C., Fung, S., Heiner, C., Bridgham, J., Chakerian, V., Heron, E., Jones, B., Menchen, S., Mordan, W., Raff, M., Recknor, M., Smith, L., Springer, J., Woo, S. and Hunkapiller, M. (1987) Biotechniques 5, 342–348.Google Scholar
  7. 7.
    Tabor, S. and Richardson, C.C. (1987) Proc. Nat. Acad. Sci. U.S.A. 84, 4767–4771.Google Scholar
  8. 8.
    Sanders, J.Z. Otto, B.J., Hood, L.E. and Smith, L. M. (unpublished data).Google Scholar

Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Lloyd M. Smith
    • 1
  1. 1.Department of ChemistryUniversity of Wisconsin-MadisonMadisonUSA

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