The Role of Active Oxygen and the Metabolism of Arachidonic Acid in the Formation of Clastogenic Factor by Human Monocytes

  • Walter Kozumbo
  • Dominique Muehlematter
  • Takafumi Ochi
  • Peter Cerutti


The mouse skin tumor promoter phorbol-12-myristate-13-acetate (PMA) is a potent inflammatory agent that not only elicits the infiltration of neutrophils, monocytes and lymphocytes to the site of application (1) but also induces human neutrophils and monocytes, but not lymphocytes, to release clastogenic factor(s) (CF) (2). CF are characterized as lipophilic (ethylacetate extractable) substances of relatively low molecular weight (<10 kDa) whose formation is mediated by superoxide anions and whose activity is inhibited by Cn superoxide dismutase (SOD), free-radical scavengers and antagonists of arachidonic acid (AA) metabolism (3). A correlation between CF formation and lipid peroxidation has been demonstrated (4). These properties of CF are suggestive of free-radical chain reactions which can give rise to lipid peroxides and possibly account for the formation and/or action of CF.


Arachidonic Acid Human Monocyte Arachidonic Acid Metabolite Mouse Embryo Fibroblast Free Arachidonic Acid 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Walter Kozumbo
    • 1
  • Dominique Muehlematter
    • 1
    • 2
  • Takafumi Ochi
    • 1
    • 2
  • Peter Cerutti
    • 1
    • 2
  1. 1.Center for Environmental MedicineUniversity of North CarolinaChapel HillUSA
  2. 2.Department of CarcinogenesisSwiss Institute for Experimental Cancer ResearchEpalinges/LausanneSwitzerland

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