Differential Effects of 12-O-Tetradecanoylphorbol-13-Acetate (TPA) on Normal Initiated and Transformed Cells of a Single Mouse Keratinocyte Lineage
Previous studies of the differential response of normal, initiated and tumor cells to TPA have been performed on cell lines derived from normal epidermis, epidermis which had been treated in vivo with chemical carcinogen, or disaggregated tumor tissue. Thus, the cell lines were heterogeneous in cellular origin. In the present study, derivatives of a single cell lineage were examined for responsiveness to TPA at various stages of tumorigenic potential after chemical treatments in vitro. The advantage of this approach is that differences in target cell response can be related to defined stages of transformation, rather than individual variation among cells of different parental origins.
KeywordsRetinoic Acid Tumor Promoter Colony Number Tumorigenic Potential Tumor Cell Derivative
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