5-Hydroxymethyluracil in Cellular DNA is Repaired and Sensitizes Cells to Inhibitors of Poly(ADP-Ribose) Synthesis

  • Robert J. Boorstein
  • Dan D. Levy
  • George W. Teebor


5-Hydroxymethyluracil (HmUra) can be formed in DNA from thymidine by the action of ionizing radiation or by activated leukocytes (1–7). HmUra can be removed from DNA in vitro through the action of HmUra-DNA glycosylase (8,9) The repairability of HmUra suggests that it is deleterious to cells but the nature of its effects on cell function are uncertain. HmUra can also be introduced into cellular DNA as a result of the incorporation of the nucleoside, 5-hydroxymethy1–2’-deoxyuridine (HmdUrd) (10–12). HmUrd has been reported to be toxic to cells in culture and to animals (11,13,14) but the mechanism of this toxicity is unknown.


Adenosine Diphosphate Ehrlich Ascites Carcinoma Cell L5178Y Cell Glycosylase Activity Diphosphate Ribose 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Robert J. Boorstein
    • 1
  • Dan D. Levy
    • 1
  • George W. Teebor
    • 1
  1. 1.Departments of Pathology and Environmental MedicineNew York University School of MedicineNew YorkUSA

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