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Plasmid Stabilization by Post-Segregational Killing

  • Kenn Gerdes
  • Jimmy Schouv Jacobsen
  • Thomas Franch
Part of the Genetic Engineering book series (GEPM, volume 19)

Abstract

Bacterial plasmids still offer some of the most versatile systems for the manipulation of genes. Bacterial plasmid-vectors have been developed for many different purposes of gene cloning. Such artificial plasmids usually consist of a replication origin, a resistance cassette and genes or expression signals for specific purposes. However, many such vectors suffer from the disadvantage of being unstably maintained. The instability of a plasmid-encoded gene will in some cases be caused by structural instability of the DNA. However, most often gene instability is caused by loss of the entire plasmid genome. In some cases, such segregational plasmid loss presents a serious practical problem either when cells are propagated in the laboratory or during optimization of gene expression.

Keywords

Plasmid Stabilization Killing Efficiency cybB Gene Severe Growth Inhibition Convenient Restriction Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Kenn Gerdes
    • 1
  • Jimmy Schouv Jacobsen
    • 1
  • Thomas Franch
    • 1
  1. 1.Department of Molecular BiologyOdense UniversityOdense MDenmark

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