Thymidine Kinase Expression

A Marker for Malignant Cells
  • Markus Hengstschläger
  • Michael Pfeilstöcker
  • Edgar Wawra
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 431)


The expression of thymidine kinase—an enzyme of the DNA precursor pathway—is strictly regulated during the normal cellular cycle, but is much higher and permanently expressed in malignant growing cells. Using a new cytofluorometric assay for thymidine kinase in single cells, we were able to discriminate between normal growing cells and virally transformed cells or lines derived from tumours. In material (blood and bone marrow) taken from leukaemia patients, we identified the leukaemic cells in a surplus of normal leucocytes. From cell cultures representing a tumour progression model, only the later, and malignant, stages showed enhanced fluorescence, whereas benign tumour cells looked normal.


Thymidine Kinase Leukaemic Cell Thymidine Kinase Activity Ewing Tumour Thymidine Kinase Expression 
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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Markus Hengstschläger
    • 1
  • Michael Pfeilstöcker
    • 2
  • Edgar Wawra
    • 1
  1. 1.Institute of Molecular BiologyUniversity of ViennaViennaAustria
  2. 2.Ludwig Boltzmann Institute for Leukaemia Research and HaematologyHanusch HospitalViennaAustria

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