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Gene Therapy for Familial Adenomatous Polyposis

Prolonged Expression of the Adenomatous Polyposis Coli Gene after Lipofection into Mouse Colon in Vivo
  • Rachel Hargest
  • Ahmed Eldin
  • Robert Williamson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 451)

Abstract

Corrective gene therapy for the treatment of single gene disorders has reached the stage of clinical trials. For malignant disease however research and clinical gene therapy protocols have focused on using genetic techniques to enhance the host’s immune response to a tumour or to direct cytotoxic therapy to tumour cells [1–3]. Corrective gene therapy has been largely ignored as a potential strategy for the treatment of malignant disease due to perceived difficulties in achieving an adequate cell hit rate [4, 5], However recent clinical [6] and preclinical studies [7, 8] have begun to address the problems raised by this strategy. Genetic modifications which reverse the malignant process could however lead to negative selection pressure followed by overgrowth of residual unmodified cancer cells. It would therefore be preferable if tumour suppressor genes could be introduced into normal tissue at a high risk of becoming malignant in order to prevent transformation, rather than reverse the neoplastic process.

Keywords

Gene Therapy Familial Adenomatous Polyposis Adenomatous Polyposis Coli Adenomatous Polyposis Coli Gene Intestinal Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

APC

adenomatous polyposis coli

FAP

familial adenomatous polyposis

MIN

murine intestinal neoplasia

PCR

polymerase chain reaction

RT PCR

reverse transcriptase polymerase chain reaction

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Rachel Hargest
    • 1
  • Ahmed Eldin
    • 1
  • Robert Williamson
    • 2
  1. 1.Department of SurgeryUniversity College London Medical SchoolLondonUK
  2. 2.Murdoch InstituteRoyal Children’s HospitalMelbourneAustralia

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