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Arterivirus PRRSV

Experimental Studies on the Pathogenesis of Respiratory Disease
  • Jörg Beyer
  • Dieter Fichtner
  • Horst Schirrmeier
  • Harald Granzow
  • Ulf Polster
  • Emilie Weiland
  • Angela Berndt
  • Helmut Wege
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

Pigs were infected with the porcine respiratory and reproductive syndrome virus (PRRSV) by the oronasal route. We studied the development of histological lesions, sites of virus infection and of inflammatory infiltrates by quantitative evaluation of reactive cells. The animals developed a multifocal interstitial pneumonia. Clinical signs of pneumonia were observed from day 7 to 21. In the first stage, an acute alveolitis was found, which was characterised by a hyperplasia of type II pneumocytes within the septa and an accumulation of macrophages in the alveolar spaces. Within 2–4 days p.i., virus infected cells were prominent in lymphatic organs, but their number declined rapidly during the following days. In the following period, the number of virus antigen positive cells increased in the lung. An interesting discrepancy existed between the relatively small number of virus specific cells and the degree of intensive pneumonia. As a first step to analyse mechanisms leading to the induction of pneumonia, we studied transcriptional expression of cytokines and other immunomodulatory molecules by semiquantitative RT-PCR.

Keywords

Alveolar Macrophage Interstitial Pneumonia Alveolar Space Transcriptional Expression Respiratory Syndrome Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Jörg Beyer
    • 1
  • Dieter Fichtner
    • 1
  • Horst Schirrmeier
    • 1
  • Harald Granzow
    • 1
  • Ulf Polster
    • 1
  • Emilie Weiland
    • 2
  • Angela Berndt
    • 3
  • Helmut Wege
    • 1
  1. 1.Federal Research Centre for Virus Diseases of AnimalsFriedrich-Loeffler-InstitutesGermany
  2. 2.Federal Research Centre for Virus Diseases of AnimalsFriedrich-Loeffler-InstitutesTübingenGermany
  3. 3.Federal Institute for Health Protection of Consumers and Veterinary MedicineJenaGermany

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