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Apoptosis of JHMV-Specific CTL in the Cns in the Absence of CD4+ T Cells

  • S. A. Stohlman
  • C. C. Bergmann
  • D. J. Cua
  • M. T. Lin
  • S. Ho
  • W. Wei
  • D. R. Hinton
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

The role of CD4+ T cells in altering the activity of cytotoxic T lymphocytes (CTL) during infection of the central nervous system (CNS) by the neuroptropic JHMV strain of mouse hepatitis virus was examined. Adoptive transfer of in vitro activated CTL into CD4-depleted and control recipients showed that CTL were not effective in reducing JHMV replication within the CNS. The distribution of CD4+ and CD8+ T cells within the CNS during JHMV infection showed that the CD4+ T cells remained in perivascular and subarachnoid spaces and few entered the parenchyma. By contrast approximately half of the CD8+ T cells entered the parenchyma. In CD4-depleted mice the trafficking of CD8+ T cells was not inhibited; however, the majority of the cells were found to be apoptotic. These data suggested that CD4+ T cells were not required for CTL induction but were required for the maintenance of CTL viability. The limited role of CD4+ T cells in CTL induction was confirmed by comparison of CTL activity from CD4-depleted and control mice.

Keywords

Adoptive Transfer None None Immunocompetent Mouse Mouse Hepatitis Virus Lymphocytic Choriomeningitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • S. A. Stohlman
    • 1
    • 2
  • C. C. Bergmann
    • 1
    • 2
  • D. J. Cua
    • 1
  • M. T. Lin
    • 3
  • S. Ho
    • 2
  • W. Wei
    • 2
  • D. R. Hinton
    • 3
  1. 1.Department of NeurologyUniversity of Southern CaliforniaLos AngelesUSA
  2. 2.Department of Microbiology and ImmunologyUniversity of Southern CaliforniaLos AngelesUSA
  3. 3.Department of PathologyUniversity of Southern CaliforniaLos AngelesUSA

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