Mouse Hepatitis Virus Nucleocapsid Protein as a Translational Effector of Viral mRNAs

  • Stanley M. Tahara
  • Therese A. Dietlin
  • Gary W. Nelson
  • Stephen A. Stohlman
  • David J. Manno
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)


The mouse hepatitis virus (MHV) nucleocapsid protein stimulated translation of a chimeric reporter mRNA containing an intact MHV 5’-untranslated region and the chloramphenicol acetyltransferase (CAT) coding region. The nucleocapsid protein binds specifically the tandemly repeated -UCYAA- of the MHV leader. This RNA sequence is the same as the intergenic motif found in the genome RNA.

Preferential translation of viral mRNA in MHV infected cells is stimulated in part by this interaction and represents a specific, positive translational control mechanism employed by coronaviruses.


Nucleocapsid Protein Intergenic Sequence Viral mRNA Chloramphenicol Acetyltransferase Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Bradford, M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of dye binding, Anal. Biochem. 72:248–254.PubMedCrossRefGoogle Scholar
  2. Cullen, B., and Malim, M. H., 1992, Secreted placental alkaline phosphatase as a eukaryotic reporter gene, Meth. Enzymol. 216:362–368.PubMedCrossRefGoogle Scholar
  3. Gorman, C. M., Moffat, L. F., and Howard, B. H., 1982, Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells, Molec. Cell. Biol. 2:1044–1051.PubMedGoogle Scholar
  4. Hilton, A., Mizzen, L., Macintyre, G., Cheley, S., and Anderson, R., 1986, Translational control in murine hepatitis virus infection, J. Gen. Virol. 67:923–932.PubMedCrossRefGoogle Scholar
  5. Kyuwa, S., Cohen, M., Nelson, G., Tahara, S. M., and Stohlman, S. A., 1994, Modulation of cellular macro-molecular synthesis by Coronavirus: implication for pathogenesis, J. Virol. 68:6815–6819.PubMedGoogle Scholar
  6. Lin, A. Y., Chang, S. C, and Lee, A. S., 1986, A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties, Molec. Cell Biol. 6:1235–1243.PubMedGoogle Scholar
  7. Nelson, G. W., 1996, [Ph.D. Dissertation]. (University of Southern California, Los Angeles, CA).Google Scholar
  8. Nelson, G. W., and Stohlman, S. A., 1993, Localization of the RNA-binding domain of mouse hepatitis virus nucleocapsid protein, J. Gen. Virol. 74:1975–1979.PubMedCrossRefGoogle Scholar
  9. Sanger, F., Nicklen, S., and Coulson, A. R., 1977, DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sei. USA 74:5463–5467.CrossRefGoogle Scholar
  10. Schneider, R., and Shenk, T., 1987, Impact of virus infection on host cell protein synthesis, Annu. Rev. Biochem. 56:317–332.PubMedCrossRefGoogle Scholar
  11. Siddell, S., Wege, H., Barthel, A., and ter Meulen, V., 1981, Intracellular protein synthesis and the in vitro translation of Coronavirus JHM mRNA, Adv. Exptl. Biol. Med. 142:193–207.Google Scholar
  12. Southern, R. J., and Berg, P., 1982, Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter, J. Mol. Appi. Gen. 1:327–341.Google Scholar
  13. Stohlman, S. A., Baric, R. S., Nelson, G. N., Soe, L. H., Welter, L. M., and Deans, R. J., 1988, Specific interaction between Coronavirus leader RNA and nucleocapsid protein, J. Virol. 62:4288–4295.PubMedGoogle Scholar
  14. Tahara, S. M., Dietlin, T. A., Bergmann, C. C, Nelson, G. W., Kyuwa, S., Anthony, R. P., and Stohlman, S. A., 1994, Coronavirus translational regulation: leader affects mRNA efficiency, Virology 202:621–630.PubMedCrossRefGoogle Scholar
  15. van Marle, G., Luytjes, W., van der Most, R. G., van der Straaten, T., and Spaan, W. J. M., 1995, Regulation of Coronavirus mRNA transcription, J. Virol. 69:7851–7856.PubMedGoogle Scholar
  16. Zhang, X., Liao, C.-L., and Lai, M. M.-C, 1994, Coronavirus leader RNA regulates and initiates subgenomic mRNA transcription both in trans and in cis, J. Virol. 68:4738–4746.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Stanley M. Tahara
    • 1
  • Therese A. Dietlin
    • 2
  • Gary W. Nelson
    • 2
  • Stephen A. Stohlman
    • 2
  • David J. Manno
    • 2
  1. 1.Department of NeurologyUSC School of MedicineLos AngelesUSA
  2. 2.Elizabeth B. Lamb Center for Pediatric ResearchVanderbilt University Medical CenterNashvilleUSA

Personalised recommendations