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Mouse Hepatitis Virus Nucleocapsid Protein as a Translational Effector of Viral mRNAs

  • Stanley M. Tahara
  • Therese A. Dietlin
  • Gary W. Nelson
  • Stephen A. Stohlman
  • David J. Manno
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 440)

Abstract

The mouse hepatitis virus (MHV) nucleocapsid protein stimulated translation of a chimeric reporter mRNA containing an intact MHV 5’-untranslated region and the chloramphenicol acetyltransferase (CAT) coding region. The nucleocapsid protein binds specifically the tandemly repeated -UCYAA- of the MHV leader. This RNA sequence is the same as the intergenic motif found in the genome RNA.

Preferential translation of viral mRNA in MHV infected cells is stimulated in part by this interaction and represents a specific, positive translational control mechanism employed by coronaviruses.

Keywords

Nucleocapsid Protein Intergenic Sequence Viral mRNA Chloramphenicol Acetyltransferase Mouse Hepatitis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Stanley M. Tahara
    • 1
  • Therese A. Dietlin
    • 2
  • Gary W. Nelson
    • 2
  • Stephen A. Stohlman
    • 2
  • David J. Manno
    • 2
  1. 1.Department of NeurologyUSC School of MedicineLos AngelesUSA
  2. 2.Elizabeth B. Lamb Center for Pediatric ResearchVanderbilt University Medical CenterNashvilleUSA

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